Figures & data
Figure 1. Structures of α-ketocaproate and α-ketoisocaproate, the best substrates of T. cruzi α-HADH-isozyme II, and the inhibitors N-propyl oxamate and N-isopropyl oxamate.
Figure 2. Effect of ethyl ester of N-propyl oxamate (Et-NPOX) (▵), ethyl ester of N-isopropyl oxamate (Et-NIPOX) (▾), and nifurtimox (○) on acute parasitemia of mice infected with INC-5 T. cruzi strain, using as a control (•) infected and non-treated mice. The drugs were administered orally 10 mg/kg per day during 60 days.
Figure 3. Effect of ethyl ester of N-propyl oxamate (Et-NPOX) (▵), ethyl ester of N-isopropyl oxamate (Et-NIPOX) (▾), and nifurtimox (○) on acute parasitemia of mice infected with NINOA T. cruzi strain, using as a control (•) infected and non-treated mice. The drugs were administered orally 10 mg/kg per day during 60 days.
Figure 4. Effect of ethyl ester of N-isopropyl oxamate (Et-NIPOX), ethyl ester of N-propyl oxamate (Et-NPOX), and nifurtimox on T. cruzi amastigote nests in myocardium of mice infected with INC-5 (hatched columns) or NINOA (open columns) T. cruzi strain. The drugs were administered orally 10 mg/kg per day during 60 days.
Figure 5. Effect of ethyl ester of N-isopropyl oxamate (Et-NIPOX), ethyl ester of N-propyl oxamate (Et-NPOX), and nifurtimox on T. cruzi amastigote nests in skeletal muscle of mice infected with INC-5 (hatched columns) or NINOA (open columns) T. cruzi strain. The drugs were administered orally 10 mg/kg per day during 60 days.
Figure 6. Histopathological studies of myocardium and skeletal muscle of mice infected with NINOA or INC-5 T. cruzi strain. (A) Myocardium from (NINOA) T. cruzi infected mice treated with the ethyl ester of N-propyl oxamate (Et-NPOX). (B) Myocardium from (NINOA) T. cruzi infected, non-treated mice. (C) Skeletal muscle from (NINOA) T. cruzi infected mice treated with Et-NPOX. (D) Skeletal muscle from (NINOA) T. cruzi infected, non-treated mice. Similar results were obtained in myocardium and skeletal muscle from (INC-5) T. cruzi infected mice, non-treated and treated with the ethyl ester of N-isopropyl oxamate (Et-NIPOX). The drugs were administered orally 10 mg/kg per day during 60 days. Tissue slices were stained with hematoxylin–eosin and were analyzed with a ×40 objective. The arrows indicate the location of amastigote nests.
