Figures & data
Figure 2. (A) Lethal factor activity as measured by fluorescence generated over time. 100 nM lethal factor, 3 μM substrate and 20 μM inhibitors were used uniformly. Assay buffer was used in place of inhibitor as a control. (B) Percent residual activity of 100 nM lethal factor and 3 μM substrate after incubation with 20 μM inhibitors. All experiments were performed in triplicate. R2 values for all data exceeded 0.99, as determined by Levenberg–Marquardt linear regression analysis, indicating a high level of goodness-of-fit.
![Figure 2. (A) Lethal factor activity as measured by fluorescence generated over time. 100 nM lethal factor, 3 μM substrate and 20 μM inhibitors were used uniformly. Assay buffer was used in place of inhibitor as a control. (B) Percent residual activity of 100 nM lethal factor and 3 μM substrate after incubation with 20 μM inhibitors. All experiments were performed in triplicate. R2 values for all data exceeded 0.99, as determined by Levenberg–Marquardt linear regression analysis, indicating a high level of goodness-of-fit.](/cms/asset/4c8774fe-0568-4af0-9d2b-a44d5895fe24/ienz_a_837901_f0002_b.jpg)