Figures & data
Figure 1. Staining SDS–PAGE (12%) gel with Coomassie brilliant blue R-250 after activity staining. The lanes are as follows: M, marker proteins with relative molecular masses indicated on the left; Lane 1, crude extract of G. stearothermophilus AH22 lipase.
![Figure 1. Staining SDS–PAGE (12%) gel with Coomassie brilliant blue R-250 after activity staining. The lanes are as follows: M, marker proteins with relative molecular masses indicated on the left; Lane 1, crude extract of G. stearothermophilus AH22 lipase.](/cms/asset/fcea48eb-fb39-4c6a-9cea-2befc89ed4bd/ienz_a_1024677_f0001_c.jpg)
Figure 2. Nondenaturing PAGE pattern of partial purified lipase. The partial purified protein was electrophoresed on 10% (w/v) polyacrylamide gel under nonreducing conditions. (A) Activity staining gel. (B) Staining gel with Coomassie brilliant blue R-250 after activity staining. Lane 1: crude extract of G. stearothermophilus AH22 lipase; Lane 2: heat treatment (30 min at 70 °C); Lane 3: pooled fractions from DEAE-Cellulose chromatography; Lane 4: pooled fractions from Sephadex G-150 chromatography; Lane 5: pooled fractions from 2nd Sephadex G-150 chromatography; Lane 6: pooled fractions from Sephadex G-25 chromatography.
![Figure 2. Nondenaturing PAGE pattern of partial purified lipase. The partial purified protein was electrophoresed on 10% (w/v) polyacrylamide gel under nonreducing conditions. (A) Activity staining gel. (B) Staining gel with Coomassie brilliant blue R-250 after activity staining. Lane 1: crude extract of G. stearothermophilus AH22 lipase; Lane 2: heat treatment (30 min at 70 °C); Lane 3: pooled fractions from DEAE-Cellulose chromatography; Lane 4: pooled fractions from Sephadex G-150 chromatography; Lane 5: pooled fractions from 2nd Sephadex G-150 chromatography; Lane 6: pooled fractions from Sephadex G-25 chromatography.](/cms/asset/732c6e14-9653-45ad-b70c-e0a9e4ee5927/ienz_a_1024677_f0002_c.jpg)
Table 1. Flowsheet of procedure used to partial purification of lipase from G. stearothermophilus AH22.
Figure 6. Effect of temperature on thermostability of AH22 lipase at room temperature, 40 and 50 °C.
![Figure 6. Effect of temperature on thermostability of AH22 lipase at room temperature, 40 and 50 °C.](/cms/asset/794d42aa-c82f-4604-9da8-f2906e4ccf0b/ienz_a_1024677_f0006_c.jpg)