3-chlorotyrosine in Sputum of COPD Patients: Relationship with Airway Inflammation

Figures & data

Table 1.  Characteristics of all patients

Patient	Age		FEV1	FEV1/FVC	Reversibility
number	Years	Sex	(% predicted)	(%)	(%)
1	76	Male	29.5	33.6	7
2	50	Female	31.6	25.3	0
3	61	Female	33.4	41.2	8
4	64	Male	29.5	32.6	6
5	78	Male	30.7	40	1
6	68	Female	34.4	39.5	9
7	67	Male	41.0	40.7	4
8	73	Male	48.9	35.2	4
9	52	Female	38.8	29.7	0
10	73	Male	50.7	61.4	3
11	66	Male	48.9	48.5	8
12	60	Female	40.7	44.4	6
13	59	Female	42.6	35.2	7
14	68	Female	26.2	32.7	10

FEV₁ –forced expiratory volume in 1 second is shown as the% of predicted for age, height and gender, FEV₁/FVC –forced expiratory volume in 1 second, expressed as a percentage of the forced vital capacity, Reversibility –increase in FEV₁ post-inhalation of 400 μg salbutamol expressed as a % of the predicted value.

Table 2.  Table of sputum characteristics, cell counts, MPO activity and 3Cl-Tyr levels for individual patients

Patient number	Sputum weight (g)	Cell viability (%)	Squamous cells (%)	Total Cell count (x10^6/g)	Neutrophils (x10^6/g)	MPO activity (μg/mL)	3Cl-Tyr (ng/mL)
1	11.1	89.7	1	2	1.8	74.15	68.4
2	5	41.2	5	1.3	1.1	21.19	5.2
3	12	91	0	1.8	1.2	32.96	2.3
4	19.3	90.2	0	2.7	2.3	76.51	10.9
5	3.5	77.2	1	5.6	4.9	47.08	9.3
6	28.3	89.4	7	3.8	2.8	44.73	11.5
7	3.6	89.3	21	2.4	na	18.83	2.6
8	5.6	98	0	26.3	24.9	174.20	27.0
9	18.5	30.4	6	0.7	0.5	10.593	1.6
10	2.2	38.5	6	0.5	0.2	10.59	0
11	23	82.8	0	14.9	11.6	23.54	2.3
12	6	26.8	5	2.5	1.5	15.30	0
13	10	59.5	3	6.5	2.8	10.59	2.1
14	4	16.5	2	4.9	1.8	11.77	2.1

MPO –myeloperoxidase activity in micrograms per millilitre, 3Cl-Tyr –3 chlorotyrosine activity in nanograms per millilitre.

Figure 1.  Showing a portion of a 3Cl-Tyr standard curve to emphasis its linear nature and LOQ. Standard curves were constructed upto 1000 ng/ml with linearity being maintained to this point

Figure 2.  A) shows a chromatograph obtained from a 0.5 ng/ml 3Cl-Tyr in PBS standard. B) shows an example chromatograph obtained from a COPD sputum sample.

Figure 3.  Correlation between MPO activity and neutrophil numbers in sputum. Each point represents one patient sample. r = 0.636, p = 0.02.

Figure 4.  Correlation of 3Cl-Tyr with neutrophil number A and MPO activity B in sputum. Each point represents one patient sample. r = 0.37 (P < 0.05) for A and 0.88 (P < 0.0001) for B.