HLA Distribution in COPD Patients

Figures & data

Table 1.  Anthropometric clinical, functional and imaging data (mean ± SD) or n (%) of participants by ATS/ERS stages of disease severity

	ATS/ERS stages
Total (N = 342)	1	2	3	4	p-value
Number of patients, n (%)	19 (5.9)	157 (49)	119 (37.2)	25 (7.8)
Age, yrs.	67.78 ± 8.45	67.97 ± 8.93	68.9 ± 7.9	63.7 ± 8.7	NS
Females, n (%)	4(21.5)	14(8.9)	2(1.6)	1(4)	NS
BMI, Kg/m2	29.05(5.2)	29.12(4.4)	27.92(4.4)	23.74(4.3)	<0.01
Smoking exp., pack-years	67.70(50)	69.2(39.29)	69.42(40.7)	64.03 (29.14)	NS
Current smokers, n (%)	4(21.05)	50(31.85)	36(30.25)	12 (48)	NS
FEV1 post-BD (% ref.)	87.36(7.38)	61.88(8.03)	41.55(4.98)	24.28(4.15)	<0.01
FEV1/FVC post-BD (%)	64.81(4.26)	59.60(8.57)	48.04(10.19)	34.23(7.19)	<0.01
DLCO (% ref.)	90.7(18.44)	70.2(18.22)	59.47(18.16)	41.73(21.62)	<0.01

BMI: body mass index; FEV1: expiratory volume during the 1st second of a forced spirometry; FVC: forced vital capacity; BD: bronchodilation; DLCO: Pulmonary diffusing capacity of carbon monoxide.

Table

	Published controls	Mallorca controls
Allele	n = 1882	AF%	n = 350	AF%	p-value
DRB1*01	205	10.89	50	14.29	0.081
DRB1*03	232	12.33	45	12.86	0.852
DRB1*04	231	12.27	36	10.29	0.336
DRB1*07	307	16.31	57	16.29	0.99
DRB1*08	51	2.71	14	4.00	0.252
DRB1*09	13	0.69	2	0.57	0.802
DRB1*10	26	1.38	4	1.14	0.918
DRB1*11	275	14.61	52	14.86	0.971
DRB1*12	21	1.12	2	0.57	0.524
DRB1*13	271	14.40	39	11.14	0.125
DRB1*14	49	2.60	10	2.86	0.928
DRB1*15	160	8.50	33	9.43	0.643
DRB1*16	41	2.18	6	1.71	0.724
Allele	n = 236	AF%	n = 252	AF%
DQB1*02	66	27.97	68	26.98	0.887
DQB1*03	70	29.66	68	26.98	0.578
DQB1*04	7	2.97	7	2.78	0.9
DQB1*05	43	18.22	52	20.63	0.576
DQB1*06	50	21.19	57	22.62	0.785

Figure 1.  Prevalence of the different DRB1 (top panel) and DQB1 (bottom panel) allelic frequencies, ordered from most to less prevalent in controls (white columns), compared to those determined in patients with COPD (grey columns).

Table 2.  Number of DRB1 and DQB1 alleles (n), allelic frequency (AF%) and Odds Ratio (OR) in controls and COPD patients

	Controls		COPD
Subjects	n = 941+175		n = 320
Allele	n = 2232	AF%	n = 640	AF%	p-value	OR	95% CI
DRB1*01	255	11.4	84	13.13	0.238	1.17	0.89–1.52
DRB1*03	277	12.4	87	13.59	0.419	1.11	0.86–1.44
DRB1*04	267	12.0	67	10.47	0.159	0.81	0.61–1.08
DRB1*07	364	16.3	96	15.00	0.463	0.91	0.71–1.16
DRB1*08	65	2.9	17	2.66	0.686	0.91	0.53–1.56
DRB1*09	15	0.7	6	0.94	0.440	1.40	0.54–3.62
DRB1*10	30	1.3	2	0.31	0.030	0.23	0.05–0.96
DRB1*11	327	14.7	80	12.50	0.177	0.83	0.64–1.08
DRB1*12	23	1.0	10	1.56	0.292	1.52	0.73–3.22
DRB1*13	310	13.9	101	15.78	0.225	1.16	0.91–1.48
DRB1*14	59	2.6	30	4.69	0.013	1.81	1.16–2.84
DRB1*15	193	8.6	49	7.66	0.468	0.88	0.64–1.21
DRB1*16	47	2.1	11	1.72	0.634	0.81	0.42–1.57
Subjects	n = 118+126		n = 320
Allele	n = 488	AF%	n = 640	AF%
DQB1*02	134	27.46	176	27.50	0.988	1.00	0.77–1.30
DQB1*03	138	28.28	188	29.38	0.737	1.06	0.81–1.37
DQB1*04	14	2.87	15	2.34	0.717	0.81	0.36–1.69
DQB1*05	95	19.47	141	22.03	0.329	1.17	0.87–1.56
DQB1*06	107	21.93	120	18.75	0.214	0.82	0.61–1.10

Table 3.  Haplotype frequency (HF%) of HLA DRB1-DQB1 haplotypes in COPD patients

DRB1	DQB1	HF%
*03	*02	13.83
*07	*02	13.40
*01	*05	12.98
*11	*03	11.49
*13	*06	11.49
*04	*03	10.00
*15	*06	6.81
*14	*05	4.68
*08	*04	2.55
*13	*03	2.55
*07	*03	2.13

Table 4.  Number of alleles (n) and DRB1 allelic frequency (AF%) in COPD patients by severity of airflow limitation (ATS/ERS stages of disease severity)

	Healthy Controls		ATS/ERS I-II			ATS/ERS III-IV
Allele	n = 2232	AF%	n = 352	AF%	p-value 1	n = 288	AF%	p-value 2
DRB1*01	255	10.9	41	11.65	0.974	43	14.93	0.107
DRB1*03	277	12.3	50	14.20	0.393	37	12.85	0.907
DRB1*04	267	12.3	42	11.93	0.987	25	8.68	0.124
DRB1*07	364	19.3	51	14.49	0.432	45	15.63	0.833
DRB1*08	65	2.7	9	2.56	0.842	8	2.78	0.898
DRB1*09	15	0.7	3	0.85	0.727	3	1.04	0.451
DRB1*10	30	1.4	1	0.28	0.112	1	0.35	0.249
DRB1*11	327	14.6	53	15.06	0.905	27	9.38	0.020
DRB1*12	23	1.1	6	1.70	0.399	4	1.39	0.541
DRB1*13	310	14.4	50	14.20	0.939	51	17.71	0.184
DRB1*14	59	2.6	11	3.13	0.733	19	6.60	0.001
DRB1*15	193	8.5	26	7.39	0.492	23	7.99	0.791
DRB1*16	47	2.2	9	2.56	0.731	2	0.69	0.065
	n = 488	AF%	n = 352	AF%	p-value	n = 288	AF%	p-value 2
DQB1*02	134	27.46	99	28.13	0.893	77	26.74	0.892
DQB1*03	138	28.28	114	32.39	0.228	74	25.69	0.486
DQB1*04	14	2.87	8	2.27	0.753	7	2.43	0.893
DQB1*05	95	19.47	73	20.74	0.713	68	23.61	0.201
DQB1*06	107	21.93	58	16.48	0.061	62	21.53	0.968

p-value 1 = controls vs. ATS/ERS I-II; p-value2 = controls vs. ATS/ERS III.-IV.

Table 5.  Number of alleles (n) and DRB1 allelic frequency (AF%) in COPD patients by DLCO impairment

	Controls		DLCO>60% ref.			DLCO≤60% ref.
Allele	n = 2232	AF%	n = 308	AF%	p-value 1	n = 244	AF%	p-value2
DRB1*01	255	11.4	42	13.64	0.299	30	12.30	0.765
DRB1*03	277	12.4	48	15.58	0.141	28	11.48	0.749
DRB1*04	267	12.0	30	9.74	0.297	30	12.30	0.962
DRB1*07	364	16.3	44	14.29	0.410	43	17.62	0.663
DRB1*08	65	2.9	7	2.27	0.652	7	2.87	0.969
DRB1*09	15	0.7	4	1.30	0.276	2	0.82	0.401
DRB1*10	30	1.3	1	0.32	0.167	1	0.41	0.358
DRB1*11	327	14.7	45	14.61	0.985	23	9.43	0.033
DRB1*12	23	1.0	3	0.97	1.0	6	2.46	0.098
DRB1*13	310	13.9	45	14.61	0.799	38	15.57	0.534
DRB1*14	59	2.6	12	3.90	0.286	15	6.15	0.004
DRB1*15	193	8.6	20	6.49	0.243	18	7.38	0.58
DRB1*16	47	2.1	7	2.27	0.849	3	1.23	0.494
	n = 488	AF%	n = 308	AF%	p-value 1	n = 244	AF%	p-value 2
DQB1*02	134	27.46	86	27.92	0.951	72	29.51	0.621
DQB1*03	138	28.28	100	32.47	0.239	62	25.41	0.463
DQB1*04	14	2.87	6	1.95	0.565	6	2.46	0.936
DQB1*05	95	19.47	66	21.43	0.568	54	22.13	0.455
DQB1*06	107	21.93	50	16.23	0.061	50	20.49	0.726

p-value1 = controls vs. DCLO>60% ref.; p-value 2 = controls vs. DCLO ≤60% ref.