The identification of a sensitizing component used in the manufacturing of an ink ribbon

Figures & data

Figure 1.  Ink mixtures used in analysis.

Table 1.  Llna phenotypic, and ige analysis after exposure to coating C.

Coating C	LLNA (DPM)	IgE + B220+		B220+		Total IgE (ng/ml)
		%	Cells × 10^6	%	Cells × 10^6
0%	482 ± 67	2.9 ± 2.0	0.2 ± 0.1	14.3 ± 1.0	0.7 ± 0.1	507 ± 66
18.75%	580 ± 84	3.5 ± 1.6	0.3 ± 0.1	16.1 ± 1.9	0.9 ± 0.2	624 ± 83
37.5%	972 ± 210	5.4 ± 1.5	0.5 ± 0.2	17.2 ± 1.7	1.2 ± 0.1	496 ± 57
75%	2644 ± 218*	10.5 ± 2.7**	1.5 ± 0.2**	27.9 ± 0.2**	3.2 ± 0.6**	713 ± 103
HCA (30%)	6199 ± 579**	Not Tested	Not Tested	Not Tested	Not Tested	Not Tested
TDI (2.5%)	Not Tested	28.0 ± 1.5**	10.5 ± 1.1**	31.2 ± 1.6**	9.3 ± 1.1**	1489 ± 186**

Levels of statistical significance are denoted as *(p = 0 05) and **(p = 001) as compared to acetone vehicle. Values present group mean (n = 5)± SE.

Figure 2  Ear swelling as a result of ink coating treatment. Analysis of irritation after topical application of (A) Coating A, (B) Coating B, and (C) Coating C. Bars represent means (± SE) of five mice (i.e., 10 ears)/group. Levels of statistical significance denoted as ** p ≤  0.01 compared to acetone vehicle.

Figure 3.  Sensitization potential following dermal treatment with ink coatings. Analysis of the sensitization potential of (A) Coating A, (B) Coating B, and (C) Coating C using the LLNA. [³H]-thymidine incorporation into draining lymph node cells of BALB/c mice following exposure to vehicle or concentrations of ink mixture shown above. Numbers appearing above bars represent stimulation indices for each concentration tested. Bars represent means (± SE) of five mice/group. Levels of statistical significance denoted as * p ≤ 0.05 compared to acetone vehicle.

Figure 4.  Sensitization potential following dermal treatment with Chemical Y. Analysis of the sensitization potential of Chemical Y using the LLNA. [³H]-thymidine incorporation into draining lymph node cells of BALB/c mice following exposure to vehicle or concentrations of Chemical Y shown above. Numbers appearing above bars represent stimulation indices for each concentration tested. Bars represent means (± SE) of five mice per group. Levels of statistical significance denoted as * p ≤  0.05 compared to acetone vehicle.

Figure 5.  Sensitization potential following dermal treatment with polyvinyl butyral. Analysis of the sensitization potential of polyvinyl butyral using the LLNA. [³H]-Thymidine incorporation into draining lymph node cells of BALB/c mice following exposure to vehicle or polyvinyl butyral. Numbers appearing above bars represent stimulation indices for each concentration tested. Bars represent means (± SE) of five mice/group. Levels of statistical significance denoted as * p ≤ 0.05 compared to acetone vehicle.