Figures & data
Figure 1. DTH responses as a result of thermal burn injury and effects of pre-/co-treatment with cimetidine. Degrees of DTH responses were significantly decreased at PBD 1, 3, 5, 10, and 14, as compared with un-burned controls (p < 0.050, 0.001, 0.0001, 0.0001, and 0.0001, respectively). Administration of cimetidine significantly mitigated effects of the burn injury on the DTH responses at PBD 5, 10, and 14 (values compared to those in injured mice that did not receive the drug; using t-test = p < 0.01, 0.001, and 0.001, respectively). * Significant difference between burn and non-burn groups. ** Significant difference between cimetidine-/non-cimetidine-treated burn mice on a given day.
Figure 2. Effects of thermal burn injury and effects of pre-/co-treatment with cimetidine, on serum levels of select cytokines. (a) Serum IL-2 levels in burned hosts were significantly lower at PBD 3, 5, 10, and 14 compared with those in non-burned controls (p < 0.004, 0.002, 0.001, and 0.020, respectively). Cimetidine treatments significantly mitigated effects of injury on IL-2 at PBD 3, 5, and 10 (values compared to those in injured mice that did not receive drug; using t-test = p < 0.050, 0.003, and 0.050, respectively). (b) Serum levels of IL-10 were significantly lower at PBD 1, 3, 5, and 10 as compared with those in unburned controls (p < 0.020, 0.004, 0.001, and 0.010, respectively). Cimetidine administration significantly augmented serum IL-10 levels at PBD 1 and 5 (values compared to those in injured mice that did not receive drug; using t-test = p < 0.05 and 0.007, respectively). Serum levels of IL-10 returned to normal at PBD 14. (c) Serum IL-12 levels were significantly lower at PBD 1, 3, 5, 10, and 14 compared with values seen in non-burn control mice (p < 0.010, 0.020, 0.003, 0.001, and 0.030, respectively). Cimetidine treatments significantly mitigated the effects of injury on IL-12 at PBD 3, 5, 10, and 14 (values compared to those in injured mice that did not receive drug; using t-test = p < 0.020, 0.001, 0.030, and 0.020, respectively). (d) Serum IL-17 levels were significantly lower at PBD 1, 3, 5, 10, and 14 as compared with those in non-burned controls (p < 0.05, 0.03, 0.04, 0.04, and 0.05, respectively). Cimetidine treatments significantly mitigated the effects of injury on IL-17 at PBD 3 and 14 (values compared to those in injured mice that did not receive drug; using t-test = p < 0.04 and 0.05, respectively). (e) Serum TGFβ levels were significantly lower at PBD 3 (p < 0.01 vs unburned control values) but were significantly higher at PBD 14 as compared with the un-burned control group (0.01). Cimetidine treatments significantly diminished the serum levels of TGF-β at PBD 14 (values compared to those in injured mice that did not receive drug; using t-test = p < 0.04). * Significant difference between burn and non-burn groups. ** Significant difference between cimetidine-/non-cimetidine-treated burn mice on a given day.
