Figures & data
Figure 1. Liver injury during APAP-induced liver toxicity. Balb/c mice were given saline or APAP (300 mg/kg) once by IP injection. At 0, 2, 6, and 24 h post-injection, mice were euthanized, blood was collected, and serum levels of ALT then measured. Results shown are the mean (± SE) of four mice.
Figure 2. Serum IL-17 levels after APAP (300 mg/kg) treatment. Results shown are the mean (± SE) of four mice (** p < 0.01).
Figure 3. APAP treatment increased levels of IL-17-producing CD4+ T-cells in the liver after 2 h. (a) CD3 and CD4 double-positive cells were gated for further analysis. (b) Example of IL-17 expression compared between control and treatment animals; the x-axis is side scatter (SSC). (c) Percentage of CD4+IL-17+ T-cells in the liver 2 h after APAP treatment compared with controls. Results shown are the mean (± SE) from three mice. Similar results were obtained in three independent experiments (p < 0.03).
Figure 4. Comparison of staining for CD1d tetramer (NKT cell marker) in total hepatic lymphocytes (left panel) and hepatic lymphocytes that stain positive for CD3/CD4/IL-17 from (right panel). The x-axis is side scatter (SSC).
