Figures & data
Table 1. Determination of isotype and affinity constant of selected monoclonal antibodies.
Figure 1. Western blot reactivity profile of selected anti-tetanus toxin monoclonal anti-bodies. Tetanus toxoid was separated over a 10% polyacrylamide gel, electroblotted to PVDF membranes, and then probed with anti-TeNT MAb. SM, protein size marker.
![Figure 1. Western blot reactivity profile of selected anti-tetanus toxin monoclonal anti-bodies. Tetanus toxoid was separated over a 10% polyacrylamide gel, electroblotted to PVDF membranes, and then probed with anti-TeNT MAb. SM, protein size marker.](/cms/asset/233b4b82-2689-4d31-a2b6-b09f15eda427/iimt_a_763872_f0001_b.jpg)
Table 2. Reactivity of specific monoclonal antibodies to tetanus toxoid, toxin, fragment C, and light chain.
Figure 2. Representative cross-inhibition profiles of tetanus toxin-specific monoclonal antibody. Increasing concentrations of competitor MAb were used to inhibit binding of the enzyme-conjugated detector MAbs 1F3E3 (a), 1F2C2 (b) and 1F4E11 (c) to tetanus toxin. The results show the percentage inhibition of the binding of detector MAb to tetanus toxoid.
![Figure 2. Representative cross-inhibition profiles of tetanus toxin-specific monoclonal antibody. Increasing concentrations of competitor MAb were used to inhibit binding of the enzyme-conjugated detector MAbs 1F3E3 (a), 1F2C2 (b) and 1F4E11 (c) to tetanus toxin. The results show the percentage inhibition of the binding of detector MAb to tetanus toxoid.](/cms/asset/078ddf0b-cbb1-4f28-9152-8aa53bb7df03/iimt_a_763872_f0002_b.jpg)
Table 3. Immunoglobulin heavy and light chain variable region genes usage by the TeNT-specific hybridoma clones.
Figure 3. Inhibition of tetanus toxin binding to ganglioside GT1b by monoclonal antibodies. An appropriate level of tetanus toxin in the presence or absence of increasing concentrations of MAb was allowed to bind to ganglioside GT1b. The percentage inhibition of TeNT biding was then calculated as described in the Materials and methods.
![Figure 3. Inhibition of tetanus toxin binding to ganglioside GT1b by monoclonal antibodies. An appropriate level of tetanus toxin in the presence or absence of increasing concentrations of MAb was allowed to bind to ganglioside GT1b. The percentage inhibition of TeNT biding was then calculated as described in the Materials and methods.](/cms/asset/0b4a38ca-8075-4885-a21d-ccb4318aec60/iimt_a_763872_f0003_b.jpg)