GST-M1 is transcribed moreso than AKR7A2 in AFB₁-exposed human monocytes and lymphocytes

Figures & data

Table 1. Designed primers sequences used in rt-qPCR.

Genes	Accession number	Forward seq. Reverse seq.	Amplicon (bp)
ACT-B	NM_001101.3	5′TGAAGATCAAGATCATTG 3′ 5′ TAACGCAACTAAGTCATA3′	179
AKR-7A2	NM_003689.2	5′GCCAGTCCGAGACCATCC3′ 5′TGCAGCCTCTTCAATGACG3′	154
AKR-7A3	NM_012067.2	5′CTATGCCTTCAACCCTCT3′ 5′CTGTTTCCCATTCTTGTCC3′	73
GST-T1	NM_000853.2	5′TCACCTTGACGGAGAGT 3′ 5′ACATCACCTTATGCCACAA 3′	174
GST-M1	NM_000561.3	5′GCAGGAAACAAGGGCTTGGA3′ 5′CCTACTTGTTGCCCCAGACA3′	103

Figure 1. AFB₁ detoxification genes expressed in human monocytes, lymphocytes, and HepG2 cells during responses to AFB₁. (Upper panel) Four AFB₁ detoxification genes and β-actin (ACT-B) mRNA in monocytes (M), lymphocytes (L), and HepG2 cells (H) were measured by RT-PCR. (Lower panel) Comparative transcription of GST-M1 and AKR-7A2 in monocytes and lymphocytes exposed to 10 or 100 ng AFB₁/ml for 2 h. All data were normalized to ACT-B internal control. Relative expression level of each gene in control monocytes and lymphocytes was set at 1.0. Data shown are means (±SEM) of cells from four individuals.

Figure 2. Cell cycle patterns of monocytes and lymphocytes after a 2-h exposure to AFB₁. The four representative histograms show the percentages of (A) lymphocytes and (B) monocytes in each cycle phase after exposure to 0, 10, or 100 ng AFB₁/ml. All X-axes are in logarithmic scale. Enumeration of the numbers of (C) lymphocytes and (D) monocytes in each cycle phase—G₀/G₀, sub-G₁ (apoptotic cells), G₂/M, and S. Open and filled bars are, respectively, geomean events/numbers, and mean events/numbers of leukocytes in 10,000 evaluated cells. Values shown are means (±SEM) of cells from four individuals. **p ≤ 0.05 versus control.