Figures & data
Figure 1. Representative dot-plots of CD19+CD27+ memory B-cells isolated by FACS. To isolate memory cells, PBMC were stained with FITC-conjugated anti-CD19 and PE-conjugated anti-CD27+ antibodies. The positive fraction was determined in comparison with isotype-matched control treated cells. Sorting gates were designed based on levels of marker expression.
Figure 2. Enumeration of EBV-transformed B-cells isolated with negative and positive MACS selection in the presence of R848 and HFF.PI6 feeder layer cells.
Table 1. Representative data for frequency of EBV transformation of B-cells isolated with negative and positive MACS selection in presence or absence of R848 and feeder layer cells.
Figure 3. Enumeration of LCL and TeNT-specific LCL-containing wells of MACS and FACS sorted memory B-cells. Results indicated significant increases in number of TeNT-specific LCL among memory B-cells isolated by FACS compared to among cells isolated by MACS.
Table 2. Representative data for frequency of LCL and TeNT-specific LCL-containing wells of MACS/FACS sorted memory B-cells transformed with EBV.
Table 3. Reactivity of anti-TeNT-specific memory B-cells with different fragments of tetanus toxin.