Genetic variation of hormone sensitive lipase and male infertility

Figures & data

Figure 1.  The PCR-RFLP products for HSL C-60G polymorphism on agarose 2%. The size of products were determined in comparison with Roche DNA molecular marker V. Homozygote CC 271 bp, homozygote GG 182 and 89 bp, and heterozygote CG 271, 182, and 89 bp.

Table 1. Basic parameters of semen sample in total infertile, asthenozoospermia, and teratoasthenozoospermia males.

Semen parameters	Infertile (n = 169)	Asthenozoospermia (n = 66)	Teratoasthenozoospermia (n = 103)
Total Sperm count (10^6)	85 ± 60	91.9 ± 72.3	79.4 ± 48.6
Sperm normal morphology (%)	17.3 ± 12	25.9 ± 14.7*	10.7 ± 4.3
Sperm motility grade a^1 (%)	2.3 ± 3.4	3.7 ± 3.8	1.3 ± 2.7
Sperm motility grade b^1 (%)	9.7 ± 7.8	13 ± 8.7	7.2 ± 5.8
Sperm motility grade c^1 (%)	13.3 ± 9.5	16.2 ± 8.6	11.2 ± 9.6
Sperm motility grade d^1 (%)	72.4 ± 21.2	67.4 ± 19	76.2 ± 22

¹ Results are presented as mean ± SD

Grade of sperm movement according to WHO [1999] criteria

a: rapid progressive, b: slow progressive, c: nonprogressive, and d: immotile

*p < 0.001 between asthenozoospermia and teratoasthenozoospermia

Table 2. The distribution of HSL genotypes (C-60G) and alleles and odds ratio of fertile (n = 164) and infertile (n = 169) males using χ2 test and regression logistics analysis.

HSL genotypes	Fertile	Infertile	Odds Ratio (95% CI)
CC	129 (78.7%)	152 (89.9%) (χ2 = 7.9, df = 1, p = 0.005)	Reference group
CG	32 (19.5%)	16 (9.5%) (χ2 = 7, df = 1, p = 0.008)	0.42 (0.22–0.81)
GG	3 (1.8%)	1 (0.6%) (χ2 = 1.3, df = 1, p = 0.25)	0.26 (0.03–2.7)
CG + GG	35 (21.3%)	17 (10.1%) (χ2 = 7.9, df = 1, p = 0.005)	0.415 (0.22–0.77)
HSL alleles
C	290 (88.4%)	320 (94.7%)	Reference group
G	38 (11.6%)	18 (5.3%) (χ2 = 8.4, df = 1, p = 0.004)	0.66 (0.49–0.88)

Overall HSL genotypes and alleles between fertile with infertile males are significantly different (χ2 = 8.14, df = 2, p = 0.017) and (χ2 = 8.34, df = 1, p = 0.004), respectively.

Table 3. Distribution and odds ratio of HSL genotypes and alleles among fertile, asthenozoospermia, and teratoasthenozoospermia males.

HSL Genotype	Fertile (n = 164)	Asthenozoospermia (n = 66)	Teratoasthenozoospermia (n = 103)	Odds Ratio (95% CI)
CC	129 (78.7%)	62 (93.2%)	90 (87.4%)	Referent group
CG + GG	35 (21.3%) a (χ2 =7.8, df = 1, p = 0.005) b ( χ2 =3.3, df = 1, p = 0.07)	4 (6.1%) c (χ2 =1.9, df = 1, p = 0.16)	13 (12.6%)	A 0.24 (0.081–0.7); B 0.53 (0.27–1.06) C 0.45 (0.14–1.4)
CC vs. CG + GG	129 vs. 35 a(χ2 =7.8, df = 1, p = 0.005) b ( χ2 =3.3, df = 1, p = 0.07)	62 vs. 4 c (χ2 =1.9, df = 1, p = 0.16)	90 vs. 13	A 4.2 (1.43–12.4) B 1.88 (0.94–3.8) C 2.24 (0.7–7.2)
HSL Allele
C	288 (88.3%)	128 (97%)	192 (93.2%)	Referent group
G	38 (11.7%) a (χ2 =3.4, df = 1, p = 0.06) b (χ2 =8.4, df = 1, p = 0.004)	4 (3%) c (χ2 = 2.3, df = 1, p = 0.13)	14 (6.8%)	A 0.49 (0.29–0.82) B 0.74 (0.54–1.02) C 0.66 (0.37–1.2)
C vs. G	288 vs. 38 a (χ2 =3.4, df = 1, p = 0.06) b(χ2 =8.4, df = 1, p = 0.004)	128 vs. 4 c (χ2 = 2.3, df = 1, p = 0.13)	192 vs. 14	A 4.42 (1.5-12.1) B 1.81(0.96–3.4) C 2.33 (0.75–7.2)

a, b: distribution of HSL genotypes and alleles between fertile with teratoasthenozoospermic and asthenozoospermic males, respectively

c: distribution of HSL genotypes and alleles between teratoasthenozoospermic and asthenozoospermic males

A, B: odds ratio (95% CI) of HSL genotypes and alleles between fertile with teratoasthenozoospermic and asthenozoospermic males, respectively

C: odds ratio (95% CI) of HSL genotypes and alleles between teratoasthenozoospermic and asthenozoospermic males