DNA fragmentation status in patients with necrozoospermia

Figures & data

Table 1. Means of age and conventional semen parameters of patients with moderate necrozoospermia, severe necrozoospermia, and controls.

	Controls Mean ± SD	Patients with Moderate necrozoospermia Mean ± SD	Patients with severe necrozoospermia Mean ± SD
Age (years)	36.76 ± 5.62	38.5 ±7.37	39.92 ± 10.23
Volume (ml)	03.20 ± 1.43	3.84 ± 2.89	2.85 ± 1.75
pH	7.74 ± 0.24	7.75 ± 0.20	7.75 ± 0.27
Necrozoospermia (%)	16.67 ± 4.40	65.67 ± 8.85 *	83.72 ± 2.79 *
Concentration (X 10^6/ml)	139.76 ± 59.97	122.34 ± 77.22	138.11 ± 31.71
Total sperm motility (%)	51.51 ± 6.36	18.32 ± 7.53 *	15.57 ± 8.43 *
Atypical forms (%)	54.33 ± 10.65	60.55 ± 1.24	59.67 ± 2.45
Multiple anomalies index	1.41 ± 0.14	1.51 ± 0.13	1.49 ± 0.19

* P < 0.05: statistically significant difference compared to controls

Figure 1.  Results of DNA fragmentation by TUNEL assay in spermatozoa from patients with normal levels of necrozoospermia, moderate necrozoospermia, and severe necrozoospermia (* p value < 0.05 compared to normozoospermic men). Patients with necrozoospermia had a high level of sperm DNA fragmentation compared to normozoospermic men with a statistically significant difference (p value < 0.05). The highest value was found in patients with severe necrozoospermia.

Figure 2.  Correlation between necrozoospermia and the sperm DNA fragmentation index. A statistically significant positive correlation was found between the degree of necrozoospermia and sperm DNA fragmentation (r = 0.878; p = 0.001).