The usefulness of Real Time Morphology software in semen assessment of teratozoospermic boars

Figures & data

Figure 1.  Comparison of two different techniques of sperm cell morphology evaluation in teratozoospermic boars. Computer assisted sperm morphology analysis using the Real Time Morphology software (RTM) and conventional assessment of semen smears stained with Giemsa stain were compared. The Bland and Altman plot assessed agreement between A) percentage of normal sperm, B) percentage of head anomalies, C) midpiece anomalies, D) proximal droplet, E) distal droplet, F) free head, G) looped tail, and H) bent tail detected by RTM and Giemsa staining. The difference in results obtained measured by the RTM and Giemsa staining methods is plotted against their average.

Table 1. Results of computer assisted sperm concentration and motility parameters analysis (n = 30) of diluted ejaculates.

	MOT (%)	PMOT (%)	VAP (μm/s)	VSL (μm/s)	VCL (μm/s)	ALH (μm)	Concentration (x10^6/ml)
mean	59.60	33.31	83.09	56.54	198.53	8.27	38.50
SD	18.99	15.05	26.60	9.76	19.70	0.82	5.69
range	13.00-82.00	3.00-58.00	15.40-117.20	32.70-69.60	157.90-237.20	7.20-9.80	32.00-52.00

MOT: total motility; PMOT: progressive motility; VAP: average path velocity; VSL: straight line velocity; VCL: curvilinear velocity; ALH: amplitude of lateral head displacement

Table 2. Percentage of morphological defects assessed by computer assisted Real Time Morphology software (RTM) and Giemsa staining method of sperm cells morphology evaluation (mead ± SD, n = 30).

		major defects				minor defects
	normal sperm	head anomalies	acrosome damage	midpiece anomalies	proximal droplet	distal droplet	free head	looped tail	bent tail
RTM	54.72 ± 7.60	1.03 ± 1.38	-	29.13 ± 10.29	5.52 ± 4.80	7.38 ± 6.69	0.42 ± 0.62	0.21 ± 0.80	1.59 ± 2.44
Giemsa staining	47.85 ± 8.81	4.44 ± 5.89	2.23 ± 2.15	29.00 ± 10.70	7.10 ± 7.15	5.97 ± 5.22	0.55 ± 0.88	0.39 ± 0.60	0.38 ± 0.93

Figure 2.  Comparison of two different techniques of sperm cell morphology evaluation in teratozoospermic boars. Computer assisted sperm morphology analysis using the Real Time Morphology software (RTM) and conventional assessment of semen smears stained with Giemsa stain. The Bland and Altman plots were used to assess the agreement between measures. Plot of the percentage of major (A) and minor defects (B) detected by the RTM and Giemsa staining. The difference in results obtained measured by the RTM and Giemsa staining methods is plotted against their average.