No association of TP53 codon 72 SNP with male infertility: a study in a Chinese population and a meta-analysis

Figures & data

Table 1. Clinical characteristics of the study subjects.

Characteristic	Case group	Control group	p
Age (years)	29.92 ± 4.76	31.48 ± 3.99	0.003
Mean sperm density (10^6/ml)	2.85 ± 1.47	64.82 ± 53.64	0.000
FSH (mIU/ml)	29.3 ± 14.53	3.7 ± 1.86	0.000
LH (mIU/ml)	7.4 ± 3.58	2.2 ± 1.32	0.000
Testosterone (ng/ml)	3.6 ± 2.31	4.5 ± 2.53	0.000

FSH: follicle-stimulating hormone; LH: luteinizing hormone.

Table 2. Analysis of association between TP53 codon 72 polymorphism and the male infertility.

	Controls (n = 401)		Cases (n = 83)
	n %		n %		OR (95% CI)	p
Recessive
Arg/Arg +Pro/Arg	311	77.60%	59	71.10%	1
Pro/Pro	90	22.40%	24	28.90%	1.41 (0.83–2.39)	0.21
Dominant
Pro/Pro+Pro/Arg	304	75.80%	59	71.10%	1
Arg/Arg	97	24.20%	24	28.90%	1.28 (0.75–2.16)	0.37
Additive
Arg	408	50.90%	83	50.00%	1
Pro	394	49.10%	83	50.00%	1.04 (0.74–1.45)	0.84

TP53: tumor protein 53; Arg: arginine allele; Pro: proline allele.

Table 3. Characteristics of case-control studies included in this meta-analysis of TP53 codon 72 polymorphism.

Male infertility group					Control group
Arg/Arg	Arg/Pro	Pro/Pro	Arg	Pro	Arg/Arg	Arg/Pro	Pro/Pro	Arg	Pro	Author (Year)	Population	HWE
36	51	23	123	97	32	95	53	159	201	Mashayekhi and Hadiyan (2012)	Caucasian	0.70
154	270	156	578	582	170	273	137	613	547	Huang et al. (2012)	Asian	0.18
136	165	60	437	285	112	180	92	404	364	Jin et al. (2013)	Asian	0.24
24	35	24	83	83	97	214	90	408	394	Chan et al. (2012)	Asian	0.35

TP53: tumor protein 53; Arg: arginine allele; Pro: proline allele; HWE: Hardy–Weinberg equilibrium.

Figure 1. Forest plot of male infertility association with distribution of TP53 codon 72 polymorphism in recessive model. A forest plot of odds ratios (OR) of male infertility for Pro/Pro when compared to Arg carriers in order of publication year was constructed. The squares and horizontal lines correspond to the OR and 95% confidence interval (CI) of each study. The center of each square represents the OR; the horizontal line shows the corresponding 95% CI of the OR. The diamond represents the pooled OR and 95% CI (OR = 0.91; 95% CI: 0.61–1.36). The pooled OR was obtained using a random-effects model and is represented by hollow diamonds, where its center indicates the OR, and its ends correspond to the 95% CI. The area of the squares reflects the relative weight of each study. The weighting factors (weight %) used to calculate the aggregate OR, calculated from the inverse of the variance, is given for each study.

Figure 2. Forest plot of male infertility association with distribution of TP53 codon 72 polymorphism in additive model. A forest plot of odds ratio (OR) of male infertility for Pro allele when compared to Arg allele in order of publication year was constructed. The squares and horizontal lines correspond to the OR and 95% confidence interval (CI) of each study. The center of each square represents the OR; the horizontal line shows the corresponding 95% CI of the OR. The diamond represents the pooled OR and 95% CI (OR = 0.86; 95% CI: 0.64–1.15). The pooled OR was obtained using a random-effects model and is represented by hollow diamonds, where its center indicates the OR, and its ends correspond to the 95% CI. The area of the squares reflects the relative weight of each study. The weighting factors (weight %) used to calculate the aggregate odds ratio, calculated from the inverse of the variance, is given for each study.

Figure 3. Forest plot of male infertility association with distribution of TP53 codon 72 polymorphism in dominant model. A forest plot of odds ratio (OR) of male infertility for Pro carriers when compared to Arg/Arg in order of publication year was constructed. The squares and horizontal lines correspond to the OR and 95% confidence interval (CI) of each study. The center of each square represents the OR; the horizontal line shows the corresponding 95% CI of the OR. The diamond represents the pooled OR and 95% CI (OR = 0.75; 95% CI: 0.51–1.11). The pooled OR was obtained using a random-effects model and is represented by hollow diamonds, where its center indicates the OR, and its ends correspond to the 95% CI. The area of the squares reflects the relative weight of each study. The weighting factors (weight %) used to calculate the aggregate odds ratio, calculated from the inverse of the variance, is given for each study.

Table 4. Meta-analysis of the association between TP53 codon 72 polymorphism and male infertility.

	Test of association	Heterogeneity	Begg's test	Egger's test
Genetic model	Pooled OR (95% CI)p	p value	p value	p value
Additive (Pro vs. Arg)	0.86(0.65–1.15)0.31	0.001	0.73	0.49
Recessive (Pro/Pro vs. Arg-carriers)	0.91(0.61–1.36)0.65	0.009	0.73	0.68
Dominant (Pro-carriers vs. Arg/Arg)	0.75(0.51–1.11)0.15	0.01	0.31	0.28

TP53: tumor protein 53; OR: odds ratio; CI: confidence interval Pro: proline allele; Arg: arginine allele.

Mashayekhi, F. and Hadiyan, S.P. (2012) A single-nucleotide polymorphism in TP53 may be a genetic risk factor for Iranian patients with idiopathic male infertility. Andrologia 44:560–564

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Huang, C., Liu, W., Ji, G.X., Gu, A.H., Qu, J.H., Song, L., et al. (2012) Genetic variants in TP53 and MDM2 associated with male infertility in Chinese population. Asian J Androl 14:691–694

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Jin, Q., Wang, B., Wang, J., Liu, T., Yu, X., Jia, C., et al. (2013) Association between TP53 gene Arg72Pro polymorphism and idiopathic infertility in southeast Chinese Han males. Syst Biol Reprod Med 59:342–346

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