Evidence for clock genes circadian rhythms in human full-term placenta

Figures & data

Figure 1. Expression of Clock mRNA in human full-term placenta at 00, 04, 08, 12, 16, and 20 hours of the day. Values are means ± SEM. Clock: circadian locomoter output cycles kaput.

Figure 2. Expression of Bmal1 mRNA in human full-term placenta at 00, 04, 08, 12, 16, and 20 hours of the day. Values are means ± SEM. Bmal: brain and muscle arnt-like.

Figure 3. Expression of Cry1 mRNA in human full-term placenta at 00, 04, 08, 12, 16, and 20 hours of the day. Values are means ± SEM. Cry: cryptochrome.

Figure 4. Expression of Per2 mRNA in human full-term placenta at 00, 04, 08, 12, 16, and 20 hours of the day. Values are means ± SEM. Per: period.

Table 1. Primers of HPRT and clock genes for RT-PCR.

Gene	Forward	Reverse
Hprt1	GGATATAAGCCAGACTTTGTTGG	CAAACATGATTCAAATCCCTGA
Clock	CAAGGAAATGTGCACTGTTGA	TATTATGGGTGGTGCCCTGT
Bmal1	TTCTCCAGGAGGCAAGAAGA	TGCTGCCTCATCATTACTGG
Per2	GTGTCAGTGTCCGGAAAAGC	GGGGCTTCATAACCAGAGTG
Cry1	CAGGCTTTTCAAGGAATGGA	ACCATTGAGTTCTATGATCTTGTCT

Hprt: hypoxanthine-guanine phosphoribosyltransferase; Clock: circadian locomoter output cycles kaput; Bmal: brain and muscle arnt-like; Per: period; Cry: cryptochrome.