17β-estradiol prevents experimentally-induced oxidative damage to membrane lipids and nuclear DNA in porcine ovary

Figures & data

Figure 1. Concentrations of malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA) in porcine ovary. Homogenates were incubated in the presence of 17β-estradiol [1 mM, 100 μM, 10 μM, 1 μM, 100 nM, 10 nM, 1 nM, 100 pM, 10 pM, 1 pM] alone (A) or in the presence of 17β-estradiol together with Fenton reaction substrates, i.e., FeSO₄ [30 μM] plus H₂O₂ [0.5 mM] (B). Data are expressed as the amount of MDA + 4-HDA (nmol) per mg of protein. Bars represent the mean ± SE of three independent experiments run in duplicates. No significant differences were found. *p<0.05 vs. Control (in the absence of both Fe²⁺+H₂O₂ and 17β-estradiol); **p<0.05 vs. Fe²⁺+H₂O₂ (in the absence of 17β-estradiol).

Figure 2. Oxidative damage to nuclear DNA in porcine ovary. DNA was incubated in the presence of 17β-estradiol [1 mM, 1 μM, 1 nM, 100 pM, 10 pM, 1 pM] alone (A) or in the presence of 17β-estradiol together with Fenton reaction substrates, i.e., FeSO₄ [30 μM] plus H₂O₂ [0.5 mM] (B). Data are expressed as the ratio 8-oxodG/dG × 10⁵. Data are from three independent experiments. Values are expressed as mean ± SE (error bars). No significant differences were found. *p<0.05 vs. Control (in the absence of both Fe²⁺+H₂O2 and 17β-estradiol); **p<0.05 vs. Fe²⁺+H₂O₂ (in the absence of 17β-estradiol).