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Mitochondrial DNA Part A
DNA Mapping, Sequencing, and Analysis
Volume 27, 2016 - Issue 2
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Mitogenome Announcement

Complete mitochondrial genome of Paramisgurnus dabryanus

, , , , &
Pages 1257-1259 | Received 22 Jun 2014, Accepted 27 Jun 2014, Published online: 04 Aug 2014
 

Abstract

In this study, the complete mitochondrial genome of Paramisgurnus dabryanus was obtained by PCR base on 18 pairs of primers. Among the 18 primers, the 14 primers were from the previously published universal primers for Cyprinus carpio L. mitogenome amplification. The remaining 4 primers were designed on the basis of related species mtDNA sequences. The genome is 16,570 bp in length, including 2 ribosomal RNA genes. 13 proteins-coding genes, 22 transfer RNA genes, and a non-coding control region, the gene composition and order of which was similar to most reported from other vertebrates. Sequence analysis showed that the overall base composition of Paramisgurnus dabryanus is T 27.3%, C 26.9%, A 28.5%, and G 17.4%. The sequence is a slight A + T bias of 55.8%, which is similar to other fishes. Mitochondrial genome is widely used in phylogenetic analysis, evolutionary genomics, species identification and related research of fish.

Declaration of interest

This study was supported by the Fuyang Science Bureau of China. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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