Abstract
We describe the mitochondrial genome of Hydropsyche pellucidula Curtis 1834, which is first described for the suborder Annulipalpia and the first in the order Trichoptera to show a non-canonical gene order. The mitogenome was obtained by de novo assembly of shotgun sequenced total genomic DNA using Illumina Miseq technology, which produced an average coverage of 115× and a minimum coverage of 48×. The mitochondrial genome includes 13 protein-coding genes, 2 rRNAs and 22 tRNAs. The genome is characterized by a rearrangement in the relative position of protein-coding and ribosomal genes. This mitogenome sequence will be useful for studying the family Hydropsychidae, which is commonly used for freshwater pollution biomonitoring.
Acknowledgments
We would like to thank Alex Aitken, Kevin Hopkins and Peter Foster (Natural History Museum), for their technical assistance and Núria Bonada and Félix Picazo for her help in morphological identification. PA, CA collected the specimen. PA performed wet lab work. BL, ACP ran the bioinformatic analyses. BL wrote the manuscript and APV supervised the project.
Declaration of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article. This work was founded by the NHM Biodiversity Initiative. PA was supported by a postdoctoral grant from The Royal Society (Newton International Program, UK).