Microsphere-embedded cryogel for selective and efficient depletion of immunoglobulin G from human serum

Figures & data

Figure 1. Illustration of MECC column for HIgG.

Table I. Swelling properties and flow resistance of cryogels.

Notations for cryogels	Monomer Conc. (%)	Yield (%)	Swelling ratio (%)	Swelling degree	Macroporosity	Flow rate (cm/h)
PHEMA	10	91	91	11.2	81	914
Composite cryogel	10	90	85	9.4	76	885

Figure 2. SEM images of control cryogel column (A) and MECC column (B).

Figure 3. Effect of flow rate on HIgG adsorption; initial HIgG concentration: 2.5 mg/mL, pH 6.0 (phosphate buffer), T: 25°C, Time: 120 min.

Figure 4. (A) Effect of initial HIgG concentration on adsorption, (B) Adsorption isotherms on MECC column. MAH content: 35.3 μmol/g, pH 6.0 (phosphate buffer), flow rate: 1 mL/min, T: 25°C.

Table II. Effect of buffer type on HIgG adsorption. MAH content: 35.3 μmol/g; HIgG concentration: 2.5 mg/mL; flow rate: 1 mL/min; T: 25°C.

Buffer type	pH	Concentration (mM)	Ionic strength	Adsorbed HIgG (mg/g)
Phosphate	6.0	20	0.010	50.5
Tris-HCl	8.0	20	0.005	69.8
MES	6.0	20	0.005	57.9

Figure 5. Effect of the ionic strength on HIgG adsorption, MAH content: 35.3 μmol/g, HIgG concentration: 2.5 mg/mL, pH 6.0 (phosphate buffer), flow rate: 1 mL/min, T: 25°C.

Figure 6. Effect of the amount of the embedded microspheres in cryogel column on HIgG adsorption. MAH content: 35.3 μmol/g, HIgG concentration: 2.5 mg/mL, pH 6.0 (phosphate buffer), flow rate: 1 mL/min, T: 25°C.

Figure 7. SDS-PAGE of serum samples before and after high-abundant protein removal. Lane 1: Protein marker. Lane 2: Crude serum. Lane 3: HIgG-depleted serum. Lane 4: Desorbed HIgG fraction. Lane 5: Standard HIgG solution.