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Research Article

Preparing a new biosensor for hypoxanthine determination by immobilization of xanthine oxidase and uricase in polypyrrole-polyvinyl sulphonate film

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Pages 327-331 | Received 11 Sep 2012, Accepted 26 Oct 2012, Published online: 10 Jan 2013

Figures & data

Figure 1. The response of Pt/PPy-PVS-XO-U electrode in different pH conditions.

Figure 1. The response of Pt/PPy-PVS-XO-U electrode in different pH conditions.

Figure 2. The response of Pt/PPy-PVS-XO-U electrode in different temperature conditions.

Figure 2. The response of Pt/PPy-PVS-XO-U electrode in different temperature conditions.

Figure 3. The response of biosensor to increasing substrate concentration.

Figure 3. The response of biosensor to increasing substrate concentration.

Figure 4. The Calibration curve for low concentration.

Figure 4. The Calibration curve for low concentration.

Figure 5. The Calibration curve for high concentration.

Figure 5. The Calibration curve for high concentration.

Figure 6. Lineweaver–Burk plot.

Figure 6. Lineweaver–Burk plot.

Figure 7. The operational stability of enzyme electrode.

Figure 7. The operational stability of enzyme electrode.

Figure 8. Storage stability of enzyme elctrode.

Figure 8. Storage stability of enzyme elctrode.

Figure 9. The calibration curve prepared by using standard addition method.

Figure 9. The calibration curve prepared by using standard addition method.

Figure 10. The hypoxanthine quantity of fish samples stored at +24°C, +6°C, −18°C.

Figure 10. The hypoxanthine quantity of fish samples stored at +24°C, +6°C, −18°C.

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