Figures & data
Figure 1. Schematic representation of Poly(ethylene glycol)-poly(lactic acid) block-copolymer encapsulated Polystroma-free hemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PEG-PLA-Poly SFHb-SOD-CAT-CA) nanocapsules.
![Figure 1. Schematic representation of Poly(ethylene glycol)-poly(lactic acid) block-copolymer encapsulated Polystroma-free hemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PEG-PLA-Poly SFHb-SOD-CAT-CA) nanocapsules.](/cms/asset/89e15c56-2435-4b23-8e42-9841bc7e46e8/ianb_a_751180_f0001_b.jpg)
Figure 2. Synthesis and characterizations of PLA-PEG (a) Chemical reaction equation to synthesize PLA-PEG: the –COOH in DL-PLA reacted with –OH in mPEG, dehydrating into PLA-PEG. (b) FTIR spectra of mPEG, PLA and PLA-PEG. 0.25 mg of the dry sample was mixed with IR-grade KBr (0.1 g) and pressed (10 ton) into tablet form. The spectrum of the tablet was then recorded by an FTIR spectrometer.
![Figure 2. Synthesis and characterizations of PLA-PEG (a) Chemical reaction equation to synthesize PLA-PEG: the –COOH in DL-PLA reacted with –OH in mPEG, dehydrating into PLA-PEG. (b) FTIR spectra of mPEG, PLA and PLA-PEG. 0.25 mg of the dry sample was mixed with IR-grade KBr (0.1 g) and pressed (10 ton) into tablet form. The spectrum of the tablet was then recorded by an FTIR spectrometer.](/cms/asset/7cc21af7-8659-4aca-9f8c-177de6cc7ef0/ianb_a_751180_f0002_b.jpg)
Figure 3. TEM pictures without metal spraying of PLA-PEG-PolyHb nanoparticles, PLA-PEG-PolySFHb nanoparticles, and PLA-PEG-PolySFHb-SOD-CAT-CA nanoparticles.
![Figure 3. TEM pictures without metal spraying of PLA-PEG-PolyHb nanoparticles, PLA-PEG-PolySFHb nanoparticles, and PLA-PEG-PolySFHb-SOD-CAT-CA nanoparticles.](/cms/asset/c77a7c5c-9d2b-4016-a1df-1599ac020fda/ianb_a_751180_f0003_b.gif)
Figure 8. Combined figure of PCO2: There were no significant differences between the PolySFHb-SOD-CAT-CA group (—□—) and the PLA-PEG-PolySFHb-SOD-CAT-CA group (—■—) in terms of PCO2 at any time of resuscitation, and they had the lower PCO2 than the other two groups. PLA-PEG-PolyHb group (—•—) had higher PCO2 after 20 mins of resuscitation than PLA-PEG-PolySFHb group (—▲—). (n = 6, P < 0.05).
![Figure 8. Combined figure of PCO2: There were no significant differences between the PolySFHb-SOD-CAT-CA group (—□—) and the PLA-PEG-PolySFHb-SOD-CAT-CA group (—■—) in terms of PCO2 at any time of resuscitation, and they had the lower PCO2 than the other two groups. PLA-PEG-PolyHb group (—•—) had higher PCO2 after 20 mins of resuscitation than PLA-PEG-PolySFHb group (—▲—). (n = 6, P < 0.05).](/cms/asset/4b7e8926-dad7-40ac-afdd-2d3153c9e253/ianb_a_751180_f0005_b.jpg)
Figure 4. The concentrations of Hb and enzymes activities in PLA-PEG-PolyHb nanoparticles, PLA-PEG-PolySFHb nanoparticles, PolySFHb-SOD-CAT-CA, and PLA-PEG-PolySFHb-SOD-CAT-CA nanoparticles.
![Figure 4. The concentrations of Hb and enzymes activities in PLA-PEG-PolyHb nanoparticles, PLA-PEG-PolySFHb nanoparticles, PolySFHb-SOD-CAT-CA, and PLA-PEG-PolySFHb-SOD-CAT-CA nanoparticles.](/cms/asset/9c5ee660-7461-4be7-91bd-02fcab6434e3/ianb_a_751180_f0006_b.jpg)
Figure 6. Combined figure of MAP: There were no significant differences between the MAP in PLA-PEG-PolySFHb group (—▲—) and PLA-PEG-PolyHb group (—•—). PLA-PEG-PolySFHb-SOD-CAT-CA group (—■—) had significantly highest MAP in all groups at any time of resuscitation, PolySFHb-SOD-CAT-CA group (—□—) had significantly lowest MAP in all groups after 15 min of resuscitation (n = 6, P < 0.05).
![Figure 6. Combined figure of MAP: There were no significant differences between the MAP in PLA-PEG-PolySFHb group (—▲—) and PLA-PEG-PolyHb group (—•—). PLA-PEG-PolySFHb-SOD-CAT-CA group (—■—) had significantly highest MAP in all groups at any time of resuscitation, PolySFHb-SOD-CAT-CA group (—□—) had significantly lowest MAP in all groups after 15 min of resuscitation (n = 6, P < 0.05).](/cms/asset/6f0d09e3-b955-4a1f-877e-5c74f5d47d9f/ianb_a_751180_f0008_b.jpg)