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Research Article

Experiment research on inhibition of glioma with sTRAIL in vitro

, , , , &
Pages 186-191 | Received 17 Dec 2012, Accepted 09 Feb 2013, Published online: 25 Oct 2013

Figures & data

Figure 1. Expression of sTRAIL gene in U251 cells. DAPI staining was also performed to visualize the apoptosis by assessing chromatin condensation. 1: Ad-sTRAIL group; 2: Ad-eGFPgroup.

Figure 1. Expression of sTRAIL gene in U251 cells. DAPI staining was also performed to visualize the apoptosis by assessing chromatin condensation. 1: Ad-sTRAIL group; 2: Ad-eGFPgroup.

Figure 2. The morphological changes under light microscope. a: normal cells(× 40); b: cells infected by Ad-sTRAIL (× 40).

Figure 2. The morphological changes under light microscope. a: normal cells(× 40); b: cells infected by Ad-sTRAIL (× 40).

Figure 3. The morphological changes under immunofluorescence microscope. a: cells infected by Ad-eGFP for 24 h; b: cells infected by Ad-eGFP for 48 h; c: cells infected by Ad-sTRAIL for 24 h; and d: cells infected by Ad-sTRAIL for 48 h.

Figure 3. The morphological changes under immunofluorescence microscope. a: cells infected by Ad-eGFP for 24 h; b: cells infected by Ad-eGFP for 48 h; c: cells infected by Ad-sTRAIL for 24 h; and d: cells infected by Ad-sTRAIL for 48 h.

Figure 4. The survival rates of U251 cells were decreased with the increase in the MOI.

Figure 4. The survival rates of U251 cells were decreased with the increase in the MOI.

Figure 5. The survival rates of U251 cells had been a sharp decline with the increase in time.

Figure 5. The survival rates of U251 cells had been a sharp decline with the increase in time.

Table I. Apoptosis rate of U251 cells after 48 h treated with virus vector (MOI = 100) (%, ± SD).

Table II. Cell cycle of U251 cells after 48 h treated with virus vector (MOI = 100) (%, ± SD).

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