An amperometric biosensor for glucose detection from glucose oxidase immobilized in polyaniline–polyvinylsulfonate–potassium ferricyanide film

Figures & data

Figure 1. Amperometric response of the Pt/Pani–Pvs, Pt/Pani–Pvs–Fc and Pt/Pani–Pvs–Mb electrodes to glucose [in the phosphate buffer (pH 7.5), 50 μL glucose oxidase (10 U/mL), 0.3 V].

Figure 2. The effect of potential on the response of the Pt/Pani–Pvs–Fc electrode to glucose [in the Phosphate buffer (pH 7.5), 50 μL glucose oxidase (10 U/mL)].

Figure 3. The effect of potassium ferricyanide concentration on the response of the Pt/Pani–Pvs–Fc electrode to glucose [in the phosphate buffer (pH 7.5), 50 μL glucose oxidase (10 U/mL), 0.3 V].

Figure 4. The effect of pH on the response of the biosensor at (25°C 5.0 × 10^{− 5} M glucose in operating potential 0.3 V).

Figure 5. The effect of temperature on the response of the biosensor at (pH 7.5, 5.0 × 10^{− 5} M glucose in operating potential 0.3 V).

Figure 6. The calibration curve of glucose biosensor (pH 7.5 phosphate buffer 25°C operating potential 0.3 V).

Figure 7. The effect of glucose concentration upon the amperometric response of the biosensor. [Lineweaver–Burk plot, in the phosphate buffer (pH 7.5) and operating potential 0.3 V, 25°C].

Figure 8. Measure number of the biosensor [in the phosphate buffer (pH 7.5), in operating potential 0.3 V, 25°C].

Figure 9. Storage stabilization of the biosensor [in the phosphate buffer (pH 7.5), in operating potential 0.3 V, 25°C and substrate concentration 5.0 × 10^{− 5} M].