Cibacron blue immobilized poly(glycidyl-methacrylate) nanobeads for albumin removal in proteome studies

Figures & data

Figure 1. AFM (A) and SEM (B) pictures of PGMA nanobeads.

Figure 2. Size distribution analysis of PGMA nanobeads.

Table I. Some physical properties of the CB-PGMA nanobeads.

Material	Initial amount of albumin (g/dL nanobeads)	Depletion of aqlbumin (mg/g nanobeads)	Depletion efficiency (% removal)
CB-PGMA nanobeads	0.4	1672	95

Figure 3. FTIR spectrum of CB, PGMA, and CB-PGMA nanobeads.

Table II. Albumin depletion efficiency of CB-PGMA nanobeads.

Physical properties	Results
Particle diameter by zeta sizer	42.5 nm
Particle diameter by AFM	45 nm
Polydispersity index	0.118
Specific surface area	2616 m^2/g
Solid density in suspension	1.049 g/mL
Amount of CB immobilization	50.3 μmol/g

Figure 4. 2D-gel electrophoresis of untreated (A) and treated (B) human plasma with CB-PGMA nanobeads.