Hematopoietic repopulating ability of CD34⁺ progenitor cells ex vivo expanded with different cytokine combinations

Figures & data

Table I. The expansion folds of total cells, CD34⁺ cells, and CFCs cultured with different cytokine combinations after 7-day culture.

Combination of cytokines	Total cells	CD34^+ cells	CFCs
STF	30.3 ± 6.4	11.7 ± 3.3	1.2 ± 0.6
STF36	39.8 ± 7.3*	4.8 ± 1.9*	0.6 ± 0.4

*P < 0.05, compared with STF group.

Table II. The number of survival NOD/SCID mice in different groups after 6 weeks.

Group	Total number of mice	Number of survival mice
Control	4	0
Fresh	4	4
STF	4	4
STF36	4	4

Control group, mice injected with the same volume IMDM; Fresh group, mice injected with freshly isolated cells; STF group, mice injected with expanded cells cultured with cytokine combination of STF; STF36 group, mice injected with expanded cells cultured with cytokine combination of STF36.

Figure 1. Level of engraftment of human CD45⁺ cells in bone marrow, spleen, and peripheral blood of recipient NOD/SCID mice transplanted with fresh CB cells or expanded cells with the cytokine combinations of STF and STF36. *p < 0.05, compared with the Fresh group. **p < 0.05, compared with the STF group.

Figure 2. An example of the expression of human ALU sequences in DNA extracted from BM cells of NOD/SCID mice engrafted with fresh and expanded CD34⁺ cells. DNA extracted from BM cells of NOD/SCID mice in control and human blood cells were used as negative (lane 1) and positive controls (lane 2), respectively. The PCR products of DNA extracted from BM cells of NOD/SCID mice in fresh cells, STF, and STF36 groups are shown in lanes 3, 4, and 5 respectively. The arrow shows that the sequence amplified by two primers is of 221 base pairs.

Table III. Human multi-lineage reconstitution in BM of NOD/SCID mice.

Groups	CD3^+CD45^+	CD19^+CD45^+	CD33^+CD45^+	CD34^+CD45^+	CD71^+CD45^+
Fresh	0.23 ± 0.21	0.19 ± 0.06	1.06 ± 0.55	24.48 ± 17.29	0.19 ± 0.02
STF	0.37 ± 0.35	12.86 ± 9.81	2.00 ± 1.78	8.35 ± 6.52	4.10 ± 3.02
STF36	0.14 ± 0.00	1.39 ± 1.73	0.23 ± 0.18	0.54 ± 0.61	0.18 ± 0.23

Table IV. Human multi-lineage reconstitution in spleen of NOD/SCID mice.

Groups	CD3^+CD45^+	CD19^+CD45^+	CD33^+CD45^+	CD34^+CD45^+	CD71^+CD45^+
Fresh	0.29 ± 0.23	0.53 ± 0.49	1.06 ± 0.33	9.09 ± 7.93	0.41 ± 0.31
STF	0.11 ± 0.08	1.16 ± 0.87	0.17 ± 0.22	0.2 ± 0.24	0.39 ± 0.48
STF36	0.35 ± 0.03	0.16 ± 0.11	0.16 ± 0.19	0.36 ± 0.26	0.23 ± 0.06

Figure 3. Colony-forming assays of bone marrow and spleen cells of recipients which were transplanted with fresh CB cells or expanded cells with the cytokine combinations of STF and STF36.