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Artificial Cells, Nanomedicine, and Biotechnology
An International Journal
Volume 44, 2016 - Issue 6
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ORIGINAL ARTICLE

Piper betle-mediated synthesis, characterization, antibacterial and rat splenocyte cytotoxic effects of copper oxide nanoparticles

Loganathan PraburamanDivision of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea
,
Jum-Suk JangDivision of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea
,
Govarthanan MuthusamyDivision of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea
,
Sengottaiyan ArumugamPG & Research Department of Biotechnology, Mahendra Arts and Science College, Kalippatti, Namakkal, Tamil Nadu, India
,
Koildhasan ManoharanDepartment of Botany, Raja Duraisingam Government Arts College, Sivagangai, Tamil Nadu, India
,
Kwang-Min ChoNational Institute of Crop Science, RDA, Iksan, Jeonbuk, South Korea
,
Cho MinDivision of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea
,
Seralathan Kamala-KannanDivision of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South KoreaCorrespondence[email protected] [email protected]
&
Oh Byung-TaekDivision of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South KoreaCorrespondence[email protected] [email protected]
 show all
Pages 1400-1405 | Received 19 Feb 2015, Accepted 10 Mar 2015, Published online: 06 Jul 2015

Figures & data

Figure 1. (a) UV–vis absorption spectrum of the CuONP synthesis using Piper betle at varying the different time intervals (0–10 h), (b) absorption spectra of CuONP synthesized at varying pH levels (4–9).
Figure 1. (a) UV–vis absorption spectrum of the CuONP synthesis using Piper betle at varying the different time intervals (0–10 h), (b) absorption spectra of CuONP synthesized at varying pH levels (4–9).
Figure 2. Transmission electron microscopy images of CuONPs.
Figure 2. Transmission electron microscopy images of CuONPs.
Figure 3. SEM–EDS spectra of CuONPs. A strong peak at 1 and 8 keV confirm the presence of Cu.
Figure 3. SEM–EDS spectra of CuONPs. A strong peak at 1 and 8 keV confirm the presence of Cu.
Figure 4. XRD pattern of CuONPs synthesized using Piper betle leaf extract.
Figure 4. XRD pattern of CuONPs synthesized using Piper betle leaf extract.
Figure 5. FTIR spectra of CuONPs synthesized from Piper betle leaf extract.
Figure 5. FTIR spectra of CuONPs synthesized from Piper betle leaf extract.
Figure 6. In vitro induction of RBC hemolysis in the presence of CuONPs (50–200 μg/mL) and control (1% TritonHX-100).
Figure 6. In vitro induction of RBC hemolysis in the presence of CuONPs (50–200 μg/mL) and control (1% TritonHX-100).
Figure 7. MTT assay for cytotoxic effect of biologically synthesized CuONPs against splenocytes at varying concentrations (0–300 μg/mL).
Figure 7. MTT assay for cytotoxic effect of biologically synthesized CuONPs against splenocytes at varying concentrations (0–300 μg/mL).

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