Propagation of human iPS cells in alginate-based microcapsules prepared using reactions catalyzed by horseradish peroxidase and catalase

Figures & data

Figure 1. Typical photomicrographs of hiPS cells enclosed in Alg-Ph microcapsules and incubated for (a) 0 (b) 8 and (c) 19 days after encapsulation (Run 1). (d) Transition of mitochondrial activity of hiPS cells enclosed in Alg-Ph microcapsules during cultivation (Runs 4–6). Scale bar: 100 μm.

Figure 2. Typical images showing encapsulated hiPS cells stained with (a, c) calcein AM dye (b, d) PI dye, to detect living and dead cells, respectively, at (a, b) 10 and (c, d) 19 days of cultivation (Run 1). Scale bar: 50 μm.

Figure 3. Typical flow cytometric histograms of unstained control and anti-SSEA-4-stained hiPS cells at (a) 9 and (b) 19 days of cultivation in Alg-Ph microcapsules (Run 1).