Figures & data
Figure 3. The effect of pH on the response of the biosensor (at 2.5 × 10−6 M ACh, at a +0.4 V operating potential).
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![Figure 3. The effect of pH on the response of the biosensor (at 2.5 × 10−6 M ACh, at a +0.4 V operating potential). Display full size 0.1 M phosphate buffer, Display full size 0.1 M glycine buffer, Display full size 0.1 M borate buffer.](/cms/asset/f95b4717-cfe0-4e97-b508-b1c4211173a4/ianb_a_1080167_f0003_c.jpg)
Figure 4. The effect of temperature on the response of the biosensor (at pH 8.0, 2.5 × 10−6 M ACh at a +0.4 V operating potential).
![Figure 4. The effect of temperature on the response of the biosensor (at pH 8.0, 2.5 × 10−6 M ACh at a +0.4 V operating potential).](/cms/asset/f5da7028-9062-4078-ae22-22a607c0213e/ianb_a_1080167_f0004_c.jpg)
Figure 5. The effect of ACh concentration on the amperometric response of the biosensor (Michaelis–Menten plot, at 0.1 M, pH 8.0 phosphate buffer, 25 °C, +0.4 V operating potential).
![Figure 5. The effect of ACh concentration on the amperometric response of the biosensor (Michaelis–Menten plot, at 0.1 M, pH 8.0 phosphate buffer, 25 °C, +0.4 V operating potential).](/cms/asset/6cbd4314-4ee0-48c5-a281-1f2d36a1b92f/ianb_a_1080167_f0005_c.jpg)
Figure 7. The effect of ACh concentration on the amperometric response of the biosensor (Lineweaver–Burk plot, at 0.1 M, pH 8.0 phosphate buffer, 25 °C, +0.4 V operating potential).
![Figure 7. The effect of ACh concentration on the amperometric response of the biosensor (Lineweaver–Burk plot, at 0.1 M, pH 8.0 phosphate buffer, 25 °C, +0.4 V operating potential).](/cms/asset/958cc1a9-9941-4495-abdb-4eb8e5c804bc/ianb_a_1080167_f0007_c.jpg)
Figure 8. Operational stability of the biosensor in pH 8.0 phosphate buffer, at a +0.4 V operating potential, 25 °C.
![Figure 8. Operational stability of the biosensor in pH 8.0 phosphate buffer, at a +0.4 V operating potential, 25 °C.](/cms/asset/b17d0ced-ffa9-41e1-abdc-4217e459c15b/ianb_a_1080167_f0008_c.jpg)
Figure 9. Storage stabilization of the biosensor pH 8.0 phosphate buffer, at a +0.4 V operating potential, 25 °C.
![Figure 9. Storage stabilization of the biosensor pH 8.0 phosphate buffer, at a +0.4 V operating potential, 25 °C.](/cms/asset/1d932c3c-f84e-4edf-a377-6db7c330dcdb/ianb_a_1080167_f0009_c.jpg)
Table I. Effect of interferants on ACh biosensor.