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Research Article

Development of an acetylcholinesterase–choline oxidase based biosensor for acetylcholine determination

, &
Pages 1659-1664 | Received 01 Apr 2015, Accepted 06 Jul 2015, Published online: 14 Sep 2015

Figures & data

Figure 1. Reaction scheme for the ACh determination.

Figure 1. Reaction scheme for the ACh determination.

Figure 2. The effect of potential on the response of the Pt/PANI-PVS electrode to H2O2.

Figure 2. The effect of potential on the response of the Pt/PANI-PVS electrode to H2O2.

Figure 3. The effect of pH on the response of the biosensor (at 2.5 × 10−6 M ACh, at a +0.4 V operating potential).

0.1 M phosphate buffer,
0.1 M glycine buffer,
0.1 M borate buffer.

Figure 3. The effect of pH on the response of the biosensor (at 2.5 × 10−6 M ACh, at a +0.4 V operating potential). Display full size 0.1 M phosphate buffer, Display full size 0.1 M glycine buffer, Display full size 0.1 M borate buffer.

Figure 4. The effect of temperature on the response of the biosensor (at pH 8.0, 2.5 × 10−6 M ACh at a +0.4 V operating potential).

Figure 4. The effect of temperature on the response of the biosensor (at pH 8.0, 2.5 × 10−6 M ACh at a +0.4 V operating potential).

Figure 5. The effect of ACh concentration on the amperometric response of the biosensor (Michaelis–Menten plot, at 0.1 M, pH 8.0 phosphate buffer, 25 °C, +0.4 V operating potential).

Figure 5. The effect of ACh concentration on the amperometric response of the biosensor (Michaelis–Menten plot, at 0.1 M, pH 8.0 phosphate buffer, 25 °C, +0.4 V operating potential).

Figure 6. The calibration curve of the ACh biosensor (at pH 8.0, 25 °C).

Figure 6. The calibration curve of the ACh biosensor (at pH 8.0, 25 °C).

Figure 7. The effect of ACh concentration on the amperometric response of the biosensor (Lineweaver–Burk plot, at 0.1 M, pH 8.0 phosphate buffer, 25 °C, +0.4 V operating potential).

Figure 7. The effect of ACh concentration on the amperometric response of the biosensor (Lineweaver–Burk plot, at 0.1 M, pH 8.0 phosphate buffer, 25 °C, +0.4 V operating potential).

Figure 8. Operational stability of the biosensor in pH 8.0 phosphate buffer, at a +0.4 V operating potential, 25 °C.

Figure 8. Operational stability of the biosensor in pH 8.0 phosphate buffer, at a +0.4 V operating potential, 25 °C.

Figure 9. Storage stabilization of the biosensor pH 8.0 phosphate buffer, at a +0.4 V operating potential, 25 °C.

Figure 9. Storage stabilization of the biosensor pH 8.0 phosphate buffer, at a +0.4 V operating potential, 25 °C.

Table I. Effect of interferants on ACh biosensor.

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