Influences of keratinocyte growth factor - mesenchymal stem cells on chronic liver injury in rats

Figures & data

Table I. Experimental groups and administration.

Groups	N	Number of injected cells or virus	Frequency
Control group	10	NS	1/week × 2 weeks, once after 2 weeks
Model group	8	NS	1/week × 2 weeks, once after 2 weeks
MSC group	10	1 × 10^6/per animal	1/week × 2 weeks, once after 2 weeks
Ad-KGF group	10	1.5 × 10^8 pfu/per animal	1/week × 2 weeks, once after 2 weeks
KGF/MSC group	10	1 × 10^6/per animal	1/week × 2 weeks, once after 2 weeks

Figure 1. MSC morphology and cell phenotypic characterization. A: MSC morphology; B: Flow cytometry results of antigen on MSC surface.

Figure 2. Determination on conditions of Ad-KGF-MSCs. A: MSC morphology after infection with different concentrations of Ad-GFP; B: KGF expression of Ad-KGF-MSCs.

Figure 3. Liver morphology and liver function after modeling. A: Rat liver morphology after modeling; B: Rat serum ALT and AST levels after modeling (** compared with control group, p<0.01).

Figure 4. Survival rate curve after treatment.

Table II. Rat body weights and liver indices after treatment (x±s).

Groups	Body weight (g)	Liver index (%)
Control group	450 ± 11.53	2.79 ± 0.23
Model group	281.3 ± 16.92***	9.34 ± 0.36***
MSC group	312.6 ± 15.37*	6.74 ± 0.52*
Ad-KGF group	341.3 ± 12.72**	7.86 ± 0.34*
KGF/MSC group	324.1 ± 15.26**	6.63 ± 1.36*

Compared with the control group, ***p<0.001; compared with the model group, *p<0.05, **p<0.01.

Table III. Rat serum liver functions after 4 weeks of treatment (x±s).

Groups	AST (U/L)	ALT (U/L)	TBILI (g/dL)	ALB (g/L)
Control group	91.45 ± 4.23	31.21 ± 3.47	4.75 ± 0.64	34.72 ± 0.87
Model group	527.35 ± 13.43***	172.54 ± 16.52***	20.74 ± 2.38***	22.45 ± 2.65***
MSC group	453.26 ± 51.72	147.04 ± 13.47	19.45 ± 3.45	25.15 ± 1.54
Ad-KGF group	434.73 ± 19.75*	161.03 ± 21.35	16.84 ± 3.11	24.87 ± 1.59
KGF/MSC group	412.25 ± 33.78**	122.26 ± 12.16*	13.46 ± 1.98**	29.41 ± 1.43*

Compared with the control group, ***p<0.001; compared with the model group, *p<0.05, **p<0.01.

Table IV. Rat liver MDA and Hyp contents (x±s).

Groups	Hyp (mg/mL)	MDA (nmol/mL)
Control group	14.55 ± 4.26	0.12 ± 0.05
Model group	98.63 ± 16.15***	1.59 ± 0.39***
MSC group	67.42 ± 15.27**	0.74 ± 0.14**
Ad-KGF group	88.32 ± 27.37	0.59 ± 0.32**
KGF/MSC group	61.15 ± 11.42**	0.48 ± 0.12**

Compared with the control group, ***p<0.001; compared with the model group, **p<0.01.

Figure 5. HE staining results of pathological sections of rat liver after treatment (100×). A: Control group; B: Model group; C: MSC group; D: Ad-KGF group; E: KGF/MSC group.