Expressions of stem cell transcription factors Nanog and Oct4 in renal cell carcinoma tissues and clinical significance

Figures & data

Figure 1. Nanog and Oct4 mRNA expressions in RCC and paracancerous tissues detected by real-time fluorescent quantitative PCR (n=86).

Table I. Correlations between Nanog and Oct4 expressions in RCC tissues and clinical pathological characteristics (n=86).

		Positive expression of protein, n (%)^a				Positive expression of mRNA, x±s^b
Characteristics	Case n (%)	Nanog	p Value	Oct4	p Value	Nanog	p Value	Oct4	p Value
Gender			0.977		0.347		0.741		0.822
Male	45 (52.3)	37 (80.4)		31 (67.4)		15.02 ± 1.04		8.97 ± 0.87
Female	41 (47.7)	32 (80.0)		26 (65.0)		15.69 ± 1.21		8.57 ± 0.85
Age/year			0.761		0.813		0.645		0.325
<60	34 (39.5)	26 (78.8)		23 (69.7)		15.79 ± 1.01		8.22 ± 0.91
≥60	52 (60.5)	43 (81.1)		34 (64.2)		15.64 ± 1.04		8.42 ± 0.94
Tumor size d/cm			0.414		0.986		0.431		0.878
<4	52 (60.5)	41 (78.8)		35 (67.3)		15.12 ± 0.92		8.39 ± 0.87
≥4	34 (39.5)	28 (82.3)		22 (64.7)		15.54 ± 0.91		8.62 ± 0.95
Histological grade			0.003*		0.034*		0.005*		0.042*
I-II	34 (39.5)	31 (88.6)		21 (60.0)		15.13 ± 1.02		8.46 ± 0.86
III-IV	52 (60.5)	38 (74.5)		36 (70.6)		16.33 ± 0.92		9.02 ± 1.05
TNM stage			0.003*		0.014*		0.025*		0.037*
I-II	35 (40.7)	26 (74.3)		20 (57.1)		15.03 ± 0.92		8.33 ± 0.87
III-IV	51 (59.3)	43 (84.3)		37 (72.5)		16.03 ± 1.02		8.99 ± 1.02
T stage			0.044*		0.057		0.075		0.102
T1-T2	40 (46.5)	29 (70.7)		27 (65.9)		15.23 ± 1.02		8.93 ± 1.05
T3-T4	46 (53.5)	40 (88.9)		30 (66.7)		15.43 ± 1.11		8.87 ± 1.01
Lymphatic metastasis			0.014*		0.164		0.390
No	62 (72.1)	48 (77.4)		40 (64.5)		15.85 ± 1.22		8.30 ± 0.95	0.475
Yes	24 (27.9)	21 (87.5)		17 (70.8)		15.98 ± 1.13		8.64 ± 1.01

^aχ² test.

^bt-test.

*p<0.05.

Figure 2. Nanog (A) and Oct4 (B) protein expressions in RCC and paracancerous tissues detected by immunofluorescent staining method.

Figure 3. Overall metastasis-free survival curves of patients with positive Nanog (A) and Oct4 (B) protein expression and negative results.

Table II. Univariate and multivariate analyses of prognostic factors.

Factor	Univariate analysis		Multivariate analysis
	p Value	HR	p Value	HR	95% CI
Tumor size	0.313	0.741	0.532	0.811	0.292-1.977
Histological grade	0.280	0.832	0.317	0.945	0.879-1.144
TNM stage	0.617	1.233	0.834	1.211	0.514-3.297
Nanog expression	0.019*	4.396	0.022*	4.411	1.215-7.707
Oct4 expression	0.075	0.097	0.087	0.092	0.904-2.311

HR: Hazard ratio; CI: Confidence interval.

*p < 0.05.