Synthesis, self-assembly, and in vitro toxicity of fatty acids-modified Bletilla striata polysaccharide

Figures & data

Figure 1. Synthesis of hydrophobically modified Bletilla striata polysaccharides with different fatty acids (m: the amount of carbon).

Figure 2. FT-IR spectra of BSP and its derivatives (a: BSP, b: hm-BSP-C₁₂, c: hm-BSP-C₁₆, d: hm-BSP-C₁₈).

Figure 3. The ¹H NMR spectra of BSP and its derivatives (a: BSP, b: hm-BSP-C₁₂, c: hm-BSP-C₁₆, d: hm-BSP-C₁₈) in deuterated DMSO.

Table 1. Properties of hm-BSPs.

Samples	DS	CMC (mg/mL)	Diameter (nm)	PI	Zeta (mv)
hm-BSP-C12	3.70	0.058	400.37 ± 1.23	0.31 ± 0.07	−0.39 ± 0.67
hm-BSP-C16	6.36	0.040	340.30 ± 6.09	0.28 ± 0.06	−0.77 ± 0.29
hm-BSP-C18	10.56	0.028	249.83 ± 8.10	0.15 ± 0.04	−0.32 ± 0.25

Figure 4. Change of the intensity ratio (I₃₇₃/I₃₉₀) from emission spectra of pyrene (6.0 × 10⁻⁷) with various concentrations of hm-BSPs in distilled water.

Figure 5. Appearance for BSP and hm-BSP solutions (a: hm-BSP-C₁₂, b: hm-BSP-C₁₆, c: hm-BSP-C₁₈, d: BSP).

Figure 6. Particle size distribution (a) and TEM photograph of hm-BSP-C₁₈ nanoparticles (b).

Figure 7. Visual observation of hemolysis caused by hm-BSP-C₁₈ at preset time intervals with different concentrations (mg/mL): (a) 0.2, (b) 0.4, (c) 0.6, (d) 0.8, (e) 1.0, (f) 1.5, (g) 2.0, (h) 4.0, (i) normal saline (negative control), and (j) distilled water (positive control).

Figure 8. Cytotoxicity of hm-BSP-C₁₈ at different concentrations against HepG2 cells for 24, 48, and 72 h. Results were shown as mean ± SD (n = 6).