Views

CrossRef citations to date

Altmetric

Articles

Determination of methemoglobin and hemoglobin levels in small volume samples

Francoise ArnaudNeuroTrauma Department, Operational and Undersea Medicine Directorate, Naval Medical Research Center, Silver Spring, MD, USA; ;Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, USACorrespondence[email protected]

Adam HigginsNeuroTrauma Department, Operational and Undersea Medicine Directorate, Naval Medical Research Center, Silver Spring, MD, USA;

Richard McCarronNeuroTrauma Department, Operational and Undersea Medicine Directorate, Naval Medical Research Center, Silver Spring, MD, USA; ;Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, USA

Paula F. Moon-MassatNeuroTrauma Department, Operational and Undersea Medicine Directorate, Naval Medical Research Center, Silver Spring, MD, USA;

Figures & data

Figure 1. A1: Sample with its native MetHb; A2: potassium cyanide (KCN) converts MetHb present in the sample to CnMetHb; A3: potassium ferricyanide (K₃FeCN₆) converts Hb present in the sample into MetHb which is converted into stable CnMetHb by KCN; A4: A1 to A4 is read at 630 nm for the MetHb; A5: Tube 4 is read at 540 nm. The difference between Tube A1 and A2 corresponds to the native MetHb from the sample; the difference between Tube A3 and A4 corresponds to the MetHb that could be present in the sample produced by all forms of Hb.

Figure 1. A1: Sample with its native MetHb; A2: potassium cyanide (KCN) converts MetHb present in the sample to CnMetHb; A3: potassium ferricyanide (K3FeCN6) converts Hb present in the sample into MetHb which is converted into stable CnMetHb by KCN; A4: A1 to A4 is read at 630 nm for the MetHb; A5: Tube 4 is read at 540 nm. The difference between Tube A1 and A2 corresponds to the native MetHb from the sample; the difference between Tube A3 and A4 corresponds to the MetHb that could be present in the sample produced by all forms of Hb.

Figure 2. Measured vs. calculated Hb from HBOC-201 samples using the ABL or 96-well-plate method. X = 96-well-plate (W) with samples diluted in buffer, ▴ 96-well-plate (W) with samples diluted in blood, and ⋄ ABL with samples diluted in buffer. Mean ± SD.

Table 1. Hb for HBOCs diluted with Sorenson’s buffer or blood

Download CSV Display Table

Table 2. MetHb (%) from samples of HBOC-201 + nitroglycerine using the 96-well-plate method compared to the ABL

Download CSV Display Table

Table 3. MetHb from OxyVita C, Sanguinate, MP4CO-NP, and M101 using the 96-well (samples diluted in buffer or blood) and the ABL compared to manufacturers’ reference values

Download CSV Display Table

**Table 4. MetHb and Hb determined using 96-well-plate method using in vivo samples from rats infused with an HBOC**

Download CSV Display Table

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Determination of methemoglobin and hemoglobin levels in small volume samples

Table 1. Hb for HBOCs diluted with Sorenson’s buffer or blood

Table 2. MetHb (%) from samples of HBOC-201 + nitroglycerine using the 96-well-plate method compared to the ABL

Table 3. MetHb from OxyVita C, Sanguinate, MP4CO-NP, and M101 using the 96-well (samples diluted in buffer or blood) and the ABL compared to manufacturers’ reference values

**Table 4. MetHb and Hb determined using 96-well-plate method using in vivo samples from rats infused with an HBOC**

Information for

Open access

Opportunities

Help and information

Your download is now in progress and you may close this window

Login or register to access this feature

Determination of methemoglobin and hemoglobin levels in small volume samples

Figures & data

Table 1. Hb for HBOCs diluted with Sorenson’s buffer or blood

Table 2. MetHb (%) from samples of HBOC-201 + nitroglycerine using the 96-well-plate method compared to the ABL

Table 3. MetHb from OxyVita C, Sanguinate, MP4CO-NP, and M101 using the 96-well (samples diluted in buffer or blood) and the ABL compared to manufacturers’ reference values

Table 4. MetHb and Hb determined using 96-well-plate method using in vivo samples from rats infused with an HBOC

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

**Table 4. MetHb and Hb determined using 96-well-plate method using in vivo samples from rats infused with an HBOC**