Gold nanoprobe-based method for sensing activated leukocyte cell adhesion molecule (ALCAM) gene expression, as a breast cancer biomarker

Figures & data

Figure 1. The absorbance spectra of synthesized AuNPs with the absorption peak at 520 nm.

Table 1. Sequences of probe, target, and negative control

Designation	Sequence (5′-3′)	Length	Tm
Probe	SH-AAAAAAAAAAAGAGCAGACGGCAGGAA	27	63.8
Target	TTCCTGCCGTCTGCTCTTCTGCCTCTTGATCTCC	34	75.2
Negative control	TGCAGGAAGTTCAGAGTTCTAGGAGTGGAAG	31	65.6

Figure 2. The absorbance spectra of synthesized AuNPs after adding NaCl with the absorption peak at 550 nm.

Figure 3. TEM micrograph of synthesized AuNPs.

Figure 4. Curve of different concentrations of gold nanoparticles and line equation of the curve.

Figure 5. The absorbance spectra of synthesized AuNPs, before and after adding MgCl₂.

Figure 6. The absorbance spectra of prepared nanoprobes, before and after adding MgCl₂.

Figure 7. The absorbance spectra of different concentrations of target, negative control and dispersion solution after adding gold nanoprobes.

Figure 8. Detection of different concentrations of ALCAM target by nanoprobes.