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Original Articles

Phages in modified alginate beads

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Pages 357-363 | Received 05 Feb 2016, Accepted 09 Feb 2016, Published online: 26 Mar 2016

Figures & data

Figure 1. Schematic drawing of the production of alginate-based beads coated also with chitosan and/or polyethyleneimine as carriers for T4 phages.

Figure 1. Schematic drawing of the production of alginate-based beads coated also with chitosan and/or polyethyleneimine as carriers for T4 phages.

Figure 2. Representative pictures of phages encapsulated within alginate beads and further coated with chitosan or PEI.

Figure 2. Representative pictures of phages encapsulated within alginate beads and further coated with chitosan or PEI.

Figure 3. Free phage activity at room temperature (A). The activity changes with time (120 min) in SGF media at 37 °C: (B) Ca+2–alginate beads; (C) Ca+2–alginate beads coated with a chitosan/alginate layer; and (D) Ca+2–alginate coated with a PEI/alginate layer.

Figure 3. Free phage activity at room temperature (A). The activity changes with time (120 min) in SGF media at 37 °C: (B) Ca+2–alginate beads; (C) Ca+2–alginate beads coated with a chitosan/alginate layer; and (D) Ca+2–alginate coated with a PEI/alginate layer.

Figure 4. Alginate beads in SIF. They do swell first and then are completely disintegrated.

Figure 4. Alginate beads in SIF. They do swell first and then are completely disintegrated.

Figure 5. Phage release from alginate-based beads carrying T4 phages: (A) Ca+2–alginate beads; (B) Ca+2–alginate beads coated with a chitosan/alginate layer; and (C) Ca+2–alginate coated with a PEI/alginate layer.

Figure 5. Phage release from alginate-based beads carrying T4 phages: (A) Ca+2–alginate beads; (B) Ca+2–alginate beads coated with a chitosan/alginate layer; and (C) Ca+2–alginate coated with a PEI/alginate layer.

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