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Original Articles

Aqueous two-phase system purification for superoxide dismutase induced by menadione from Phanerochaete chrysosporium

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Pages 380-388 | Received 30 Nov 2015, Accepted 26 Feb 2016, Published online: 29 Mar 2016

Figures & data

Table 1. Effects of molecular weight of PEG and system composition in PEG–K2HPO4 on menadione induced SOD partitioning at 25 °C

Table 2. Effects of molecular weight of PEG and system composition in PEG–K2HPO4 on menadione-induced SOD partitioning at 25 °C

Table 3. Effects of PEG 3350 concentration and system pH on menadione induced SOD partitioning at 25 °C

Table 4. Effects of system pH on menadione induced SOD partitioning at 25 °C

Figure 1. (A) Elution profile of SOD from Sephadex G-100 column; (B) Molecular weight calibration curve; standard proteins (–•–) and ATPS purified SOD (–○–).

Figure 1. (A) Elution profile of SOD from Sephadex G-100 column; (B) Molecular weight calibration curve; standard proteins (–•–) and ATPS purified SOD (–○–).

Figure 2. SDS–PAGE profiles of P. chrysosporium SOD purified by partitioning in ATPS. Lane 1: molecular weight markers (15–260 kDa) (10 μg), lane 2: top phase of the optimized system (25 μg), lane 3: crude SOD extract (25 μg) and lane 4: bottom phase of the optimized system (25 μg).

Figure 2. SDS–PAGE profiles of P. chrysosporium SOD purified by partitioning in ATPS. Lane 1: molecular weight markers (15–260 kDa) (10 μg), lane 2: top phase of the optimized system (25 μg), lane 3: crude SOD extract (25 μg) and lane 4: bottom phase of the optimized system (25 μg).

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