Protective effects of Trifolium alexandrinum L. against lung injury induced by environmental toxin CCl₄ in experimental rats

Figures & data

Table 1 Effect of TAME on the protein and lung marker enzymes of rats

Treatment	Protein (µg/mg tissue)	CAT (U/min)	POD (U/min)
Control	280.7±9.6^++	1.7±0.4^++	3.1±0.5^++
CCl4	187.4±13.2**	0.9±0.2**	1.7±0.3**
CCl4+silymarin	277.8±10.6^++	1.5±0.3^++	3.0±0.6^++
CCl4+TAME	230.5±12.3^++	1.1±0.1^++	2.7±0.5^++

Mean±SEM (n=6).

**Indicate significance from the control group at p<0.01 probability level.

⁺⁺Indicate significance from the CCl₄ group at p<0.01 probability level.

Table 2 Effect of TAME on GSH-Px and GST in rat lungs

Treatment	GST (nmol/min/mg protein)	GSH (mol/g tissue)	GSH-Px (mol/g tissue)
Control	34.9±3.2^++		110.8±5.3^++
CCl4	23.3±2.5**	13.93±1.4^++	99.7±4.8**
CCl4+silymarin	34.5±2.7^++	8.14±0.9**	112.2±6.3^++
CCl4+TAME	28.7±3.1^++	13.78±1.3^++	108.1±5.7^++

Mean±SEM (n=6).

**Indicate significance from the control group at p<0.01 probability level.

⁺⁺Indicate significance from the CCl₄ group at p<0.01 probability level.

Fig. 1 Effects of TAME on TBARS in rat lungs.

Table 3 Effect of TAME on the body and lung weight of rats

Treatment	Initial body wt (g)	Final body wt (g)	% increase body wt	Absolute lungs wt	Relative lungs wt
Control	190.3±5.7^++	198.2±5.2^++	4.2±0.7^++	1.5±0.5^++	0.75±0.05^++
CCl4	185.2±6.2**	192.3±9.0**	3.8±0.5**	1.9±0.2**	0.98±0.07**
CCl4+silymarin	185.7±6.1^++	195.8±7.8^++	5.4±0.9^++	1.5±0.4^++	0.76±0.04^++
CCl4+TAME	185.4±7.3^++	194.6±5.7^++	4.8±0.5^++	1.5±0.3^++	0.77±0.09^++

Mean±SEM (n=6).

** Indicate significance from the control group at p<0.01 probability level.

⁺⁺Indicate significance from the CCl₄ group at p<0.01 probability level.