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Original Research Articles

Swedish isolates of Vibrio cholerae enhance their survival when interacted intracellularly with Acanthamoeba castellanii

, PhD, , MD, MSc, Assistant Professor, , PhD, Assistant Professor, , PhD, Professor & , PhD, Researcher
Article: 31060 | Received 20 Jan 2016, Accepted 23 Feb 2016, Published online: 25 Apr 2016

Figures & data

Fig. 1 Viable counts of V. cholerae in the absence of A. castellanii. V. cholerae O3 (♦), O4 (▴), O5 (□), O11 (▪) and O160 (●). Data represent means ±SD of three independent experiments.

Fig. 1 Viable counts of V. cholerae in the absence of A. castellanii. V. cholerae O3 (♦), O4 (▴), O5 (□), O11 (▪) and O160 (●). Data represent means ±SD of three independent experiments.

Fig. 2 Viable counts of V. cholerae in the presence of A. castellanii. V. cholerae O3 (♦), O4 (▴), O5 (□), O11 (▪), and O160 (●). Data represent means ±SD of three independent experiments.

Fig. 2 Viable counts of V. cholerae in the presence of A. castellanii. V. cholerae O3 (♦), O4 (▴), O5 (□), O11 (▪), and O160 (●). Data represent means ±SD of three independent experiments.

Fig. 3 Intracellular growth and survival of V. cholerae. Viable counts of intracellular V. cholerae O3 (♦), O4 (▴), O5 (□), O11 (▪), and O160 (●). Data represent mean ±SD of three independent experiments.

Fig. 3 Intracellular growth and survival of V. cholerae. Viable counts of intracellular V. cholerae O3 (♦), O4 (▴), O5 (□), O11 (▪), and O160 (●). Data represent mean ±SD of three independent experiments.

Fig. 4 Electron microscope image showing the intracellular localisation of V. cholerae in A. castellanii. A. A. castellanii trophozoite in the absence of bacteria. B. V. cholerae O160 localised in cytoplasmic vacuoles of A. castellanii trophozoite, 2 h after co-cultivation. C. V. cholerae O4 in cytoplasmic vacuoles of A. castellanii trophozoite, 4 h after co-cultivation.

Fig. 4 Electron microscope image showing the intracellular localisation of V. cholerae in A. castellanii. A. A. castellanii trophozoite in the absence of bacteria. B. V. cholerae O160 localised in cytoplasmic vacuoles of A. castellanii trophozoite, 2 h after co-cultivation. C. V. cholerae O4 in cytoplasmic vacuoles of A. castellanii trophozoite, 4 h after co-cultivation.

Fig. 5 Growth of A. castellanii. Viable counts of A. castellanii in the absence of V. cholerae strains (⋄), and in the presence of V. cholerae O3 (♦), O4 (▴), O5 (□), O11 (▪), and O160 (●). Data represent mean ±SD of three independent experiments.

Fig. 5 Growth of A. castellanii. Viable counts of A. castellanii in the absence of V. cholerae strains (⋄), and in the presence of V. cholerae O3 (♦), O4 (▴), O5 (□), O11 (▪), and O160 (●). Data represent mean ±SD of three independent experiments.