First TBEV serological screening in Flemish wild boar

Figures & data

Fig. 1 Map of Wild boar sampling (left) and TBEV-seropositives (right) in Flanders. Left Part: Study population and positives per community; Right Part: Calculated wild boar TBEV-seroprevalence based on 10 SNT-reactors (positive/borderline – cut-off 1/10) out of 238 wild boar tested. FLA: Flanders total study population (n=238); WFL: West Flanders subpopulation (n=77); LIM: Limburg+Antwerp subpopulation (n=161).

Table 1 Confirmation panel of 10 TBEV-reactors in SNT and/or ELISA.

	TBEV	TBEV	TBEV	LIV	CSFV	USUV	WNV	WNV	DENV	YFV	JEV
Sample No.	IgG ELISA (VIEU/ml)	SNT (titre)	IFA (titre)	HIT (titre)	ELISA/SNT	90%PRNT (titre)	90%PRNT (titre)	IFA (titre)	1–4 IFA (titre)	IFA (titre)	IFA (titre)
1	POS	POS	NEG	NEG	NEG	NEG	NEG	NEG	NEG	NEG	NEG
	230	1/33	<1/10	<1/20		<1/20	<1/20	<1/10	<1/10	<1/10	<1/10
2	POS	POS	POS	border	NEG	NEG	NEG	NEG	NEG	NEG	NEG
	>540	1/164	1/1,000	1/20		<1/20	<1/20	<1/10	<1/10	<1/10	<1/10
3	POS	POS	POS	POS	NEG	/	/	NEG	NEG	NEG	NEG
	490	1/243	1/3,200	1/40				<1/10	<1/10	<1/10	<1/10
4	POS	POS	POS	POS	/	NEG	NEG	NEG	NEG	NEG	NEG
	520	1/127	1/3,200	1/40		<1/20	<1/20	<1/10	<1/10	<1/10	<1/10
5	POS	NEG	NEG	NEG	NEG	NEG	NEG	NEG	NEG	NEG	NEG
	>540	<1/10	<1/10	<1/20		<1/20	<1/20	<1/10	<1/10	<1/10	<1/10
6	POS	NEG	/	border	/	/	/	/	/	/	/
	430	<1/10	/	1/20		/	/	/	/	/	/
7	POS	NEG	NEG	NEG	/	NEG	NEG	NEG	NEG	NEG	NEG
	265	<1/10	<1/10	<1/20		<1/20	<1/20	<1/10	<1/10	<1/10	<1/10
8	NEG	POS	NEG	POS	NEG	POS	POS	NEG	NEG	NEG	NEG
	15	1/140	<1/10	1/80		1/320	1/390	<1/10	<1/10	<1/10	<1/10
9	NEG	POS	NEG	border	NEG	NEG	NEG	NEG	NEG	NEG	NEG
	35	1/17	<1/10	1/20		<1/20	<1/20	<1/10	<1/10	<1/10	<1/10
10	NEG	POS	NEG	NEG	/	NEG	NEG	NEG	NEG	NEG	NEG
	15	1/25	<1/10	<1/20		<1/20	<1/20	<1/10	<1/10	<1/10	<1/10

TBEV-reactors in TBEV-SNT (n=7/10) and reactors in TBEV-ELISA (n=3); n=7 samples reacted. TBEV: tick-borne encephalitis virus; LIV: louping ill virus; CSFV: classical swine fever virus; USUV: Usutu virus; WNV: West Nile virus; DENV: dengue virus; YFV: yellow fever virus; JEV: Japanese encephalitis virus; IgG: immunoglobulin of subtype G; ELISA: enzyme-linked immunosorbent assay; SNT: seroneutralisation test; IFA: immunofluorescence assay; HIT: haemagglutination inhibition test; PRNT: plaque reduction neutralisation test; Bold=positive result, not bold=negative or borderline result, NEG: negative result; /: no result due to bad quality or lack of volume sample.