Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in diarrhoeic patients in the Qikiqtani Region, Nunavut, Canada

Figures & data

Fig. 1.  Map of Qikiqtani Region of Nunavut, Canada, © Government of Canada, Source: Library and Archives Canada's website (www.collectionscanada.ca).

Table I. Detection of Cryptosporidium and Giardia infections in diarrhoeic patients from Nunavut (n=108) by polymerase chain reaction (PCR) and microscopy

	No. samples positive
Parasites	PCR	Microscopy	PCR and/or Microscopy^a	% of patients positive
Cryptosporidium spp.	17	8	17	15.7
Giardia duodenalis	5	3	5	4.6
Total	22	11	22	20.3

^aPatients were considered infected if their stool sample was positive by either PCR or microscopy, or both.

Fig. 2.  Phylogenetic analysis of Cryptosporidium parvum gp60 sequence data using neighbour-joining analysis. Sequences from the present study (3C, 9C, 1C, 18C, 2C, 65C and 17C) as well as reference sequences representing C. parvum sub-genotypes (acquired from GenBank) are indicated. Evolutionary distances were computed using the Kimura 2-parameter method. Bootstrap values>75% from 1,000 replicates are shown.

Table II. Microsatellite analysis of Cryptosporidium gp60 subtypes in diarrhoeic patients from Nunavut (n=7)

Species	Genotypes	Sub-genotypes	No. patients
Cryptosporidium	IIa	A15G2R1	3
parvum	IIa	A15G1R1	2
	IIa	A16G1R1	1
	IIa	A15R1	1