Binding properties of Treponema denticola lipooligosaccharide

Figures & data

Table 1 Binding of tritium-labeled T. denticola LOS to human ECM proteins immobilized in the wells of a microplate

Protein	Bound T. denticola LOS (ng)
Type I collagen	17±9
Type IV collagen	ND
Fibronectin	37±12
Laminin	357±22

ND, not detected.

Table 2 Binding of tritium-labeled T. denticola LOS to gingival epithelial cells and fibroblasts in the presence and absence of soluble CD14

	Bound T. denticola LOS (ng)
Amount of soluble CD14 (ng/ml)	Epithelial cells	Fibroblasts
0	137±14	159±24
10	141±32	212±84
20	139±28	359±62*
40	203±71	422±49*

*Significant increase (P < 0.01) compared to the control without soluble CD14.

Table 3 Binding of tritium-labeled T. denticola LOS to bacteria immobilized in the wells of a microplate

Bacteria	Bound T. denticola LOS (ng)
A. viscosus ATCC 27044	31±12
P. micros 1251	452±37
P. micros 1262	404±31
P. gingivalis ATCC 33277	ND
S. mitis ATCC 33399	33±9
S. mutans ATCC 25175	54±15
S. salivarius ATCC 25975	94±29
S. sanguinis NY101	ND

ND, not detected.

Table 4 Cytokine secretion by human gingival fibroblasts stimulated with T. denticola LOS-coated P. micros cells.

	Amount of cytokine secreted (pg/ml)
Stimulation condition	IL-6	IL-8
Control	ND	31±9
P. micros	91±32	165±26
T. denticola LOS-coated P. micros	437±75*	594±91*
T. denticola LOS-coated P. micros+polymyxin B	123±42	245±84

ND, not detected.

*Significant increase (P < 0.01) compared to the stimulation with P. micros alone.