Genotoxic effect induced by hydrogen peroxide in human hepatoma cells using comet assay

Figures & data

Figure 1.  Levels of DNA damage after exposure to hydrogen peroxide evaluated by the comet assay.

Figure 2.  Flowchart showing the comet assay for single-cell gel electrophoresis to determine DNA damage (12).

Figure 3.  Comet assay of the effect of duration of incubation with hydrogen peroxide on DNA damage: untreated HepG2 cells (A) and HepG2 cells treated with 50 µM H₂O₂ for 5 min (B), 1 h (C) or 24 h (D). Longer tails indicate more damage.

Figure 4.  Dose and time dependence of the extent of DNA damage. HepG2 cells were treated with 25 µM or 50 µM H₂O₂ for 5, 30, 40 min, 1 h or 24 h and DNA damage was assessed by the comet assay. Damage is expressed in olive tail moment (OTM). The values are the mean±SEM of 50 cells from two pooled wells (25 cells per slide). ^* p<0.001 (One-Way ANOVA) compared to control.