Correlations between Lumbricus terrestris survival and gut microbiota

Figures & data

Fig. 1.  Earthworm survival curves. The color of the curves indicate feeding regime. The significance of the difference between the groups was determined by the Breslow-Gehan test.

Table 1. DNA sequence of the MCR components

Name	DNA sequence (5′-3′)
Flavobacteriaceae	GCAAGCGTTATCCGGATTTATCTGGGTGTAAAGGGAGCSTAGARGGTTTGTTCAAGTCATGATGTGAYTCTCTCTGCGGCTCACCTGTGGAAACTGCATGTTGATACTGTGATTAGTCTTGAGTGRAGTWGGAGGAGGCGATCGTGAGTGMTAGGCGTTGGAATTGCGCGTATAGGAATATATTCAGCGAGAGACACTCAGATTGTGGCGAGGCGCTCACTAGAAGCTAGTA
Ruminococcaceae	CGAGCGTTATCCGGATTATCTGGCGTAAAGGCCGTAGCGTTAGTAGTCTAGTGTGAATCCAGTGGCTCAACCTTGGACTGCATTGATACTGCTGAGAACTATGAGTTCATGTGAGAGGTAGACGTGGATTCCGAMTGAGTGTAGCGGTGAATGCGTAGAGTATTTCASGAGARACCAGATGGCGAAGCGCGAGCTCTCATCTGCATCAAGTGATGA
Lachnospiraceae	AAGCGTTATCCGATTATCTGGGCGTAAAGCSATGTAGGCGTTTACTAAGTCTGGTGTGAAAGCTCACGGCCTAACCGTGAATTGCATTGGAAACTGGTGAACTAGAATACTGGAGGGGTAAGTGGAATTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAGGAACACCAATGGCGAAGCGCAGCTTACTGGACAGAGA
Ferrimonadaceae	AGCGTTAGATTCGGATTACTGGGCGTAAAGCGCACSAGCGTTTGGWTAAGTTCAGATGTGAAATGCCCCGGGCTCAACCTGTGGARTGCATTTAGAATACTGTCCAGCTATGGAGTCTTGTYGAGGGGGGTAGAATTCTCAGGTGTAGCGGTGATGCGTAGAGATCTGAGAATCACCGGTGTGCGAAGGCGGCCCCCTGACAAATGA
Oceanospirillaceae	GCGAGSGGGTGAAGGGCCTGTGTATATCTGGCGTAAAACGCGCCCTCTAGCGGTTTTATTAAGTCTGGGTGAAATCCGGGCCTCAACCTTGGGACTGCTTTGAAACTGGGGCTGAGTTCGGGAGYGGAAGTGGATTCCTGTGTAGCGGTGAAATTGCGTAGATATTCTGGAGGACACCAGTGGCGAAGCGGCTCTACTGCCGTAC

Table 2. Classification of MCR components by the RDP hierarchical classifier^a

MCR components^b	Domain	Phylum	Class	Order	Family
Flavobacteriaceae is	Bacteria	Bacteroidetes	Flavobacteria	Flavobacteriales	Flavobacteriaceae
Ruminococcaceae is	Bacteria	Firmicutes	Clostridia	Clostridiales	Ruminococcaceae
Lachnospiraceae is	Bacteria	Firmicutes	Clostridia	Clostridiales	Lachnospiraceae
Ferrimonadaceae is	Bacteria	Proteobacteria	γ-proteobacteria	Alteromonadales	Ferrimonadaceae
Oceanospirillaceae is	Bacteria	Proteobacteria	γ-proteobacteria	Oceanospirillales	Oceanospirillaceae

^aBold indicate the bootstrap classification support >50%.

^bThe naming of the MCR components is based on the family names. Since none have bootstrap support >50% the classification is incertae sedis(is).

Fig. 2.  Gut microbiota clustering based on the concentration profiles for the MCR components. The concentration of each of the components in each sample is indicated by the color code. The clustering represents the relatedness based on the centroide linkage.

Fig. 3.  PLS regressions with the gut microbiota as predictors. (A) Live/dead correlations, (B) correlations to cellulase activity, and (C) correlations to feeding regime. The regression coefficients with standard deviations are shown.