Coryneform bacteria in human semen: inter-assay variability in species composition detection and biofilm production ability

Figures & data

Table 1 Identification of the coryneform strains

Strain	16S rRNA ID	%	Latter primer ID	%	API Coryne	ID that was used for biofilm analysis
CM7	Actinomyces neuii subs. neuii	100	Actinomyces neuii subs. anitratus	100	Not analyzed	Actinomyces neuii
CM2	Arthrobacter cumminsii	100	Arthrobacter cumminsii	99	Cellulomonas/Microbacterium	Arthrobacter cumminsii
10040G	Arthrobacter sp	96	Dermabacter hominis	100	Cellulomonas/Microbacterium	Dermabacter hominis
10040R1	Arthrobacter sp.	97	Dermabacter hominis	99	C. seminale	Dermabacter hominis
03019A	Brevibacterium sp.	97	Brevibacterium lutescens	100	Corynebacterium sp.	Brevibacterium lutescens
04016D	Brevibacterium sp.	98	Uncultured bacterium clone ncd2467c01c1	99	C. propinquum	Brevibacterium sp.
01007A	C. aurimucosum/nigricans	98	Uncultured bacterium	100	Corynebacterium group G	C. nigricans/aurimucosum
SP9F	C. aurimucosum	99	Uncultured bacterium	99	Corynebacterium group G	C. aurimucosum
CM12-1	C. freneyi	96	C. freneyi	99	C. striatum/amycolatum	C. freneyi
02005P1	C. seminale ^$	99	C. seminale	99	C. seminale	C. seminale
03016B2	C. seminale	99	C. seminale	100	C. seminale	C. seminale
03026B	C. seminale	99	C. seminale	100	C. seminale	C. seminale
SP12B2	C. seminale	99	C. seminale	96	C. seminale	C. seminale
CM12-4	C. seminale	100	C. seminale	99	not analyzed	C. seminale
02016C	C. glutamicum	91	Uncultured bacterium	100	Could not be identified	C. glutamicum ^€
CM21	C. hansenii	96	C. amycolatum	99	Not analyzed	C. amycolatum
01007Q	C. minutissimum	98	Uncultured bacterium	100	Could not be identified	C. minutissimum
SP16B	C. minutissimum	89	C. minutissimum	98	Corynebacterium group G	C. minutissimum
CM17	C. minutissimum	98	Uncultured bacterium	99	Not analyzed	C. minutissimum
CM18	C. minutissimum	98	Uncultured bacterium	100	Not analyzed	C. minutissimum
CM14	C. ureicelerivorans	93	Uncultured bacterium	100	Not analyzed	C. ureicelerivorans ^€
SP41C	Dermabacter hominis	100	Dermabacter hominis	99	Dermabacter hominis	Dermabacter hominis
12034F1	Dermabacter hominis	99	Dermabacter hominis	99	Dermabacter hominis	Dermabacter hominis
SP6C	Zimmermannella alba/Pseudoclavibacter sp.	97	Agrococcus terreus/Leifsonia naganoensis	99	C. afermentans/coyleae	Zimmermannella alba/Pseudoclavibacter sp./Agrococcus terreus/Leifsonia naganoensis ^¥

^$Synonym of Corynebacterium glucuronolyticum.

^€The estimated likelihood that this species is identified correctly is less than 96%. Interpret with caution.

^¥A coryneform strain with identification signature that discourages suggesting a particular species.

Table 2 Biofilm production of coryneform bacteria

		Biofilm OD (extinction at 630 nm)
Species	Number of strains	Range	Mean±SD	Number of biofilm producers
Arthrobacter cumminsii	1	0.327	0.327	1
Actinomyces neuii	1	0.296	0.296	1
Brevibacterium lutescens	2	0.063–0.299	0.181±0.167	1
C. amycolatum	1	0.093	0.093	0
C. aurimucosum	1	0.065	0.065	0
C. freneyi	1	0.113	0.113	0
C. seminale ^$	5	0.057–0.091	0.070±0.014	0
C. minutissimum	4	0.066–0.247	0.120±0.085	1
C. nigricans/aurimucosum	1	0.072	0.072	0
C. ureicelerivorans	1	0.072	0.072	0
Dermabacter hominis	4	0.085–0.482	0.300±0.170	3
Zimmermannella alba/Pseudoclavibacter sp./Agrococcus terreus/Leifsonia naganoensis ^¥	1	0.063	0.063	0
C. glutamicum ^€	1	0.069	0.069	0
Subject group	Number of strains	Range	Mean±SD	Number of biofilm producers
Healthy	12	0.057–0.299	0.142±0.100	3
Prostatitis	12	0.063–0.482	0.154±0.150	4

^$Synonym of C. glucuronolyticum.

^¥A coryneform strain with identification signature that discourages suggesting a particular species.

^€The estimated likelihood that this species is identified correctly is less than 96%. Interpret with caution.