Chemical constituents and antioxidant activity of the Arctic mushroom Lycoperdon molle Pers

Figures & data

Fig. 1  Free-radical scavenging (FRS) activity of the Arctic mushroom Lycoperdon molle in various solvents: methanol, ethanol, acetone, dimethyl sulfoxide (DMSO), butylated hydroxytoluene (BHT); and butylated hydroxyanisol (BHA).

Fig. 2  Inhibition of lipid peroxidation (ILP) activities of the Arctic mushroom Lycoperdon molle in various solvents: methanol, ethanol, acetone, dimethyl sulfoxide (DMSO), butylated hydroxytoluene (BHT); and butylated hydroxyanisol (BHA).

Table 1 Antioxidant activity, polysaccharide, protein and total polyphenol content of Lycoperdon molle extracts in various solvents. Data presented are the average of three consecutive readings of the extract in each assay system.

		Antioxidant activities			Polysaccharide content (mg/ml)	Protein content (µg/ml)	Total polyphenol content (mg/ml)
Solvent extracts	Extract dry wt. g	FRS^1 (%)	ILP^2 (%)	TEAC^3 (mM)
Acetone	0.06	57.87	39.00	7.4	14.92	24.0	0.031
Ethanol	0.08	87.27	45.00	7.0	14.85	8.04	0.090
Methanol	0.64	89.60	54.41	–	14.68	8.63	0.304
DMSO^4	0.11	44.00	32.00	2.4	4.00	8.0	0.325

¹Free-radical scavenging activity.

²Inhibition of lipid peroxidation.

³Trolox equivalent antioxidant capacity.

⁴Dimethyl sulfoxide.

Fig. 3  Electrospray ionization mass spectrometry profile of fraction A in the negative mode.

Fig. 4  Electrospray ionization mass spectrometry profile of fraction A in the positive mode.

Fig. 5  Electrospray ionization mass spectrometry/tandem mass spectra of m/z 540.7.