Effects of Mannan Oligosaccharide and Inulin on Sharpsnout Seabream (Diplodus Puntazzo) in the Context of Partial Fish Meal Substitution by Soybean Meal

Figures & data

Table 1. Fatty acid profile of the diets.

	Control diet	Experimental diets
	FM	SBM	SBM-MOS	SBM-INU
Fatty acids, g/100 g of total fatty acids
14:0	6.3		7.6
15:0	0.4		0.4
16:0	18.8		18.6
17:0	0.3		0.3
18:0	5.4	4.8
20:0	0.2		0.1
14:1	0.3		0.3
16:1 n-7	6.6		6.6
17:1	0.1		0.1
18:1 n-9	16.4		17.0
22:1 n-11	7.0		6.1
24:1 n-9	0.7		0.5
18:2 n-6	13.0		11.4
18:3 n-3	1.8		1.5
18:4 n-3	3.6		5.5
20:3 n-3	0.4		0.2
20:4 n-6	0.9		0.7
20:5 n-3	5.5		6.1
22:5 n-3	1.2		1.0
22:6 n-3	11.2		11.3
Total saturated	32.2		31.7
Total monounsaturated	31.1		30.6
Total polyunsaturated	37.6		37.7
Total n-3	23.7		25.6
Total n-6	13.9		12.1
n-3/n-6	1.7		2.1

SBM, diet with approximately 40% of the protein supplied by soybean meal; SBM-MOS, diet with addition of 8 g of mannanoligosaccharide (MOS); SBM-INU, diet with addition of 8 g of inulin (INU).

Table 2. Growth parameters of sharpsnout seabream (n=3 per treatment) fed the experimental diets.

	FM	SBM	SBM-MOS	SBM-INU	SEM	P
Initial weight, g	101.3	96.5	96.7	100.6	28.2	0.588
Final weight, g	238.1	234.2	229.8	235.4	104.9	0.798
Weight gain, g	136.8	137.7	133.1	134.8	50.2	0.830
SGR	0.60	0.59	0.58	0.57	0.006	0.968
DIR	1.11	1.12	1.11	1.10	0.008	0.994
FCR	2.06	2.01	2.05	2.06	0.009	0.909
PER	1.00	1.02	1.00	1.00	0.004	0.970

FM, control diet; SBM, diet with approximately 40% of the protein supplied by soybean meal; SBM-MOS, diet with addition of 8 g of mannanoligosaccharide (MOS); SBM-INU, diet with addition of 8 g of inulin (INU); SGR, specific growth rate; DIR, daily intake rate; FCR, feed conversion ratio; PER, protein efficiency ratio.

Table 3. Effect of different diets on sharpsnout seabream somatic parameters and body composition (g/100 g wet wt).

	FM	SBM	SBM-MOS	SBM-INU	SEM	P
Condition factor	2.01	2.02	2.01	2.01	0.013	0.993
Viscerosomatic index	6.14^a	5.89^b	5.51^b	5.53^b	0.290	0.046
Hepatosomatic index	1.35^a	1.02^b	1.13^b	1.19^ab	0.079	0.005
Mesenteric fat index	1.85^a	1.72^ab	1.43^b	1.49^b	0.128	0.012
Moisture	68.9^b	69.9^b	72.7^a	72.3^a	0.580	0.001
Crude protein	17.0	16.4	16.3	16.9	0.142	0.125
Crude fat	8.0^a	7.9^a	5.8^b	6.1^b	0.165	0.001
Ash	4.3^a	3.9^ab	3.6^b	3.7^b	0.025	0.003

FM, control diet; SBM, diet with approximately 40% of the protein supplied by soybean meal; SBM-MOS, diet with addition of 8 g of mannanoligosaccharide (MOS); SBM-INU, diet with addition of 8 g of inulin (INU).

^a,bValues on the same line with different superscripts are significantly different (P<0.05).

Table 4. Fatty acid profiles of total lipids in fillets of Diplodus puntazzo from different diets (g of fatty acid/100 g of total fatty acids).

	FM	SBM	SBM-MOS	SBM-INU	SEM	P
14:0	7.3^b	7.7^b	7.2^b	8.5^a	0.065	0.001
15:0	0.4^a	0.4^a	0.2^b	0.2^b	0.001	0.001
16:0	19.5^c	19.4^c	22.2^a	20.5^b	0.532	0.005
17:0	0.2^b	0.2^b	0.3^a	0.3^a	0.002	0.014
18:0	4.7^a	3.8^b	4.7^a	4.0^b	0.053	0.021
20:0	0.1	0.1	0.1	0.1	0.002	0.809
14:1	0.4^a	0.4^a	0.1^b	0.1^b	0.001	0.001
16:1 n-7	7.0	6.6	6.7	6.6	0.053	0.182
17:1	0.1	0.1	0.1	0.1	0.003	0.989
18:1 n-9	15.8^b	15.9^b	18.4^a	17.3^ab	0.608	0.010
22:1 n-11	8.1^a	6.5^b	6.5^b	7.0^b	0.053	0.001
24:1 n-9	0.4	0.4	0.3	0.4	0.001	0.144
18:2 n-6	7.7	8.4	7.0	7.3	0.575	0.206
18:3 n-3	1.5	1.5	1.3	1.7	0.078	0.429
18:4 n-3	6.4^ab	6.8^ab	6.1^b	6.9^a	0.076	0.027
20:3 n-3	0.2^a	0.1^b	0.2^a	0.2^a	0.001	0.002
20:4 n-6	0.6	0.6	0.6	0.6	0.019	0.975
20:5 n-3	9.1^a	8.9^a	6.2^b	6.3^b	0.352	0.003
22:5 n-3	0.6	0.6	0.6	0.6	0.016	0.995
22:6 n-3	9.9	11.6	11.2	11.3	0.564	0.098
Total saturated	32.2	31.3	34.7	33.6	1.445	0.051
Total monounsaturated	31.8	30.2	32.1	31.5	1.350	0.170
Total polyunsaturated	36.0^ab	38.5^a	33.2^b	34.9^b	1.511	0.004
Total n-3	27.7^ab	29.5^a	25.6^b	27.0^b	0.740	0.003
Total n-6	8.3	9.0	7.6	7.9	0.315	0.070
n-3/n-6	3.34	3.28	3.37	3.42	0.102	0.979
AI	0.72	0.73	0.72	0.82	0.007	0.456
TI	0.28	0.26	0.31	0.29	0.002	0.591

FM, control diet; SBM, diet with approximately 40% of the protein supplied by soybean meal; SBM-MOS, diet with addition of 8 g of mannanoligosaccharide (MOS); SBM-INU, diet with addition of 8 g of inulin (INU); AI, atherogenic index; TI, thrombogenic index.

^a,bValues on the same line with different superscripts are significantly different (P<0.05).