Effect of Monochromatic and Combined Light Colour on Performance, Blood Parameters, Ovarian Morphology and Reproductive Hormones in Laying Hens

Figures & data

Figure 1. Effect of LED light colours on the follicle stimulating hormone (FSH) levels in laying hens at 22 weeks of age.

Figure 2. Effect of LED light colours on 17β-estradiol levels in laying hens at 22 weeks of age.

Figure 3. Effect of LED light colours on serum immunoglobulin-G (IgG) concentrations in laying hens at 22 weeks of age.

Figure 4. Effect of LED light colours on the heterophil:lymphocyte ratio (H:L) in laying hens at 22 weeks of age.

Table 1. Effect of LED light colours on the performance (22-57 weeks) of laying hens.

	Treatment						SEM	P
	R	G	B	W	R→G	R→G→B
Egg production, %
22-30 weeks	77.84^ab	75.63^bc	74.05^c	75.39^bc	79.66^a	77.18^ab	0.43	0.01
31-39 weeks	92.59^ab	90.63^bc	89.64^c	91.14^bc	93.31^a	93.16^a	0.29	0.002
40-48 weeks	91.57^ab	89.54^bc	87.57^c	89.86^bc	92.19^a	92.09^a	0.33	0.002
49-57 weeks	90.16^a	87.45^b	85.87^b	88.23^ab	90.83^a	89.08^a	0.39	0.03
Mean	87.79^ab	85.82^bc	84.28^c	86.16^bc	88.99^a	87.88^ab	0.41	0.004
Egg weight, g
22-30 weeks	60.01^bc	60.86^ab	61.87^a	59.85^bc	59.57^bc	60.62^abc	0.22	0.02
31-39 weeks	62.47^bc	62.37^ab	63.64^a	62.34^bc	63.01^abc	62.80^abc	0.14	0.03
40-48 weeks	62.79^b	64.94^a	65.39^a	63.35^b	63.20^b	62.88^b	0.34	0.003
49-57 weeks	62.91	64.99	65.87	64.24	63.92	63.51	0.38	0.08
Mean	61.76^b	63.06^a	63.63^a	61.85^b	61.93^b	62.10^b	0.17	0.004
Egg mass, g
22-30 weeks	46.72	46.03	45.81	45.12	47.46	46.79	0.27	0.32
31-39 weeks	57.86	57.43	57.04	56.82	58.79	58.51	0.21	0.07
40-48 weeks	57.49	58.15	57.27	56.92	58.26	57.91	0.19	0.08
49-57 weeks	56.71	56.82	56.56	56.68	58.05	56.57	0.23	0.13
Mean	54.70	54.61	54.17	53.89	55.64	54.95	0.44	0.61
Feed intake, g/d
22-30 weeks	129.50	130.83	128.54	129.14	130.16	131.39	0.37	0.62
31-39 weeks	132.83	132.15	132.75	131.88	132.97	132.41	0.32	0.45
40-48 weeks	134.45	134.85	135.56	137.64	133.87	135.67	0.36	0.14
49-57 weeks	139.02	139.64	138.73	139.47	139.97	139.14	0.46	0.39
Mean	134.08	134.28	133.90	134.53	133.99	134.66	0.22	0.44
Feed conversion rate
22-30 weeks	2.77	2.84	2.81	2.86	2.74	2.81	0.02	0.473
31-39 weeks	2.29	2.30	2.33	2.32	2.26	2.26	0.01	0.152
40-48 weeks	2.34	2.32	2.37	2.42	2.29	2.34	0.01	0.064
49-57 weeks	2.45	2.47	2.46	2.47	2.41	2.46	0.01	0.056
Mean	2.46^bc	2.48^abc	2.49^ab	2.51^a	2.42^c	2.47^bc	0.01	0.041

R, red light; G, green light; B, blue light; W, white light.

^a,b,cValues with the same letters in a row are not significantly different (P>0.05).

Table 2. Effect of different LED light colours on egg quality in laying hens at 24 weeks of age.

	Treatment						SEM	P
	R	G	B	W	R→G	R→G→B
Shell colour	22.48^a	21.97^b	21.39^b	21.56^b	23.01^ab	22.54^ab	0.23	0.03
Albumin height, mm	9.83	9.75	10.33	10.34	10.09	9.90	0.17	0.98
Haugh unit	96.51	97.01	99.88	100.24	99.25	97.86	0.78	0.93
Yolk colour	6.47	6.42	6.54	6.44	6.26	6.39	0.04	0.72
Egg shell breaking strength, kg/cm^2	4.71	4.83	4.28	4.48	4.48	4.74	0.07	0.72

R, red light; G, green light; B, blue light; W, white light.

^a,bValues with the same letters in a row are not significantly different (P>0.05).

Table 3. Effect of different LED light colours on ovarian parameters of laying hens at 22 weeks of age.

	Treatment						SEM	P
	R	G	B	W	R→G	R→G→B
BWSM, g	1748.67^bc	1777.01^ab	1836.67^a	1734.67^bc	1691.00^c	1773.67^ab	7.33	0.02
ASM, d	143.67^d	154.67^ab	158.67^a	154.01^ab	147.33^cd	151.00^bc	1.03	0.005
BMW, g	51.13^c	75.10^ab	78.47^a	63.60^abc	55.77^bc	73.23^ab	3.12	0.04
LW, g	41.33	33.93	36.53	38.90	42.57	35.50	1.30	0.50
SW, g	3.0	2.57	3.10	3.06	3.97	2.83	0.37	0.77
OW, g	54.53^a	42.27^c	43.23^c	45.27^bc	49.93^ab	50.27^ab	0.86	0.002
StW, g	3.30^a	2.30^c	2.43^bc	2.82^abc	3.27^a	3.50^a	0.11	0.02
OvW, g	53.99	51.25	49.57	50.03	54.24	53.00	0.92	0.19
TLYFW, g	32.03	27.97	26.80	30.01	35.17	26.97	0.84	0.19
UPOFW, g	42.10	38.27	39.23	39.87	40.02	37.00	0.62	0.49
Follicle
1-3 mm	14.33^a	11.0^bc	9.33^c	10.33^bc	15.66^a	14.67^a	0.53	0.003
4-6 mm	11.33^a	7.0^2bc	6.04^c	8.01^bc	9.67^ab	8.67^abc	0.42	0.02
7-9 mm	2.67	2.0	1.33	2.00	2.33	2.0	0.18	0.82
10 mm and above	7.67	6.33	5.33	7.33	6.0	5.67	0.38	0.20

R, red light; G, green light; B, blue light; W, white light; BWSM, body weight at sexual maturity; ASM, age at sexual maturity; BMW, breast muscle weight; LW, liver weight; SW, spleen weight; OW, ovary weight; StW, stromal weight; OvW, oviduct weight; TLYFW, total large yellow follicle weight; UPOFW, unexplained post-ovulatory follicle weight.

^a-dValues with the same letters in a row are not significantly different (P>0.05).

Table 4. Effect of LED light colours on the biochemical blood parameters of laying hens at 22 weeks of age.

	Treatment						SEM	P
	R	G	B	W	R→G	R→G→B
ALT, U/L	2.74	1.66	0.93	1.80	2.31	2.69	0.33	0.16
GLU, mg/dL	306.89^ab	307.53^ab	279.99^c	285.72^b	305.64^ab	323.13^a	4.24	0.029
T P, g/dL	7.35	7.95	7.65	8.08	8.13	9.30	0.20	0.35
T-CHOL, mg/dL	174.75	194.50	183.25	167.0	191.75	208.50	8.29	0.19
ALB, g/dL	2.45	2.70	2.75	2.63	2.73	3.0	0.05	0.13
TG, mg/dL	1309.0^a	978.3^bc	936.8^c	1000.7^bc	1231.3^ab	1053.8^abc	33.22	0.037

R, red light; G, green light; B, blue light; W, white light; ALT, alanine aminotransaminase; GLU, glucose; TP, total protein; ALB, albumin; T-CHOL, total cholesterol; TG, triglycerides.

^a,b,cMean values within a row followed by the same letter are not significantly different (P>0.05).