Proteomic Analysis of a 14.2 kDa Protein Isolated from Bali Cattle (Bos Sondaicus/Javanicus) Saliva Using Single Dimension Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Time-of-Flight Tandem Mass Spectrometer

Figures & data

Figure 1. Commassie brilliant blue stain one-stained single dimension 12.5% sodium dodecyl sulphate-polyacrylamide gel electrophoresis image of a 14.2 kDa (arrow, lane 1) protein isolated from Bali cattle (Bos sondaicus/javanicus) saliva. The band (indicated by the arrow) was sliced and analysed using matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometer with peptide mass fingerprinting searching of database. Molecular marker masses (kDa) are represented in lane 2.

Table 1. Mascot search results of protein spot (single dimension 12.5%, sodium dodecyl sulphate-polyacrylamide gel electrophoresis of a 14.2 kDa isolated from Bali cattle saliva) identification by matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry.

Mascot hit number	Protein sequence hit ID	Score	Nominal mass - Mr, kDa	Calculated pI	Protein sequence coverage, %	Accession blast	Description	Maximum identity, %
1	G3MZ19	425	17.708	8.52	48	gi|296473588|D AA15703.1 gi|359079571|X P_002697975.2	HRPE773-like (Bos taurus) Predicted zymogen granule protein 16 homologue B (Bos taurus)	100
2	F1N1Z8	131	22.091	9.41	13	gi|296473587|D AA15702.1	Pancreatic adenocarcinoma upregulated factor-like (Bos taurus)	100
3	F1MCV8	79	9.007	8.96	25	gi|124249234|N P_001074382.1	Prolactin-inducible protein homologue precursor (Bos taurus)	99

ID, identification sequence; Mr, relative molecular mass; pI, isoelectric point value.