Histomorphometrical Evaluation of Gastrointestinal Tract and Performance of Ross Broilers Hatched from Eggs Inoculated with Salmonella Enteritidis Phage Type 4

Figures & data

Table 1. Relative weight and length of the intestine of Ross chicks at days 1, 7, 14 and 21 of age.

	1 d		7 d		14 d		21 d
	I, g/100 g of live weight	IL, cm	I, g/100 g of live weight	IL, cm	I, g/100 g of live weight	IL, cm	I, g/100 g I, g/100 g weight	IL, cm
Contamination site
Shell	4.18	39.87	8.78	84.92	6.29	101.50	4.86	108.25
Internal	3.61	39.00	9.22	76.62	7.22	97.50	5.47	116.50
Inoculated agent
SE	4.00	39.00	8.82	87.92^a	7.38	97.75	6.17^a	114.87
NC	3.78	39.87	9.19	76.62^b	6.13	101.50	4.16^b	109.87
Variation factor, %
V	ns	ns	ns	ns	ns	ns	ns	ns
A	ns	ns	ns	ns	ns	ns	ns	ns
V ×A	ns	ns	ns	ns	ns	ns	ns	ns
CV, %	19.3	4.93	6.35	3.56	11.31	4.65	48.50	45.19

I, relative weight; IL, length; V, contamination site: internal, allantoidal cavity; A, inoculated agent; SE, Salmonella Enteritidis; NC, negative control (0.85% sterilised buffered saline); CV, coefficient of variation; ns, not significant.

^a,bDifferent letters in the same column and line differ statistically in Tukey’s test.

Table 2. Villus height and crypt depth of duodenum of Ross chicks at days 1 and 21 of age after inoculation.

	1 d		21 d
VH, µm	CD, µm	VH, µm	CD, µm
Contamination site
Shell	369.60	69.67	1327.71^a	106.35
Internal	226.11	70.99	1165.98^b	99.61
Inoculated agent
SE	280.1	76.8	1241.1	106.0^a
NC	414.1	63.9	1352.6	84.1^b
Variation factor, %
V	0.01	ns	0.001	ns
A	0.01	ns	ns	0.001
V ×A	0.01	0.13	ns	ns
CV, %	27.77	24.86	18.84	28.63

VH, villus height; CD, crypt depth; V, contamination site: internal, allantoidal cavity; A, inoculated agent; SE, Salmonella Enteritidis; NC, negative control (0.85% sterilised buffered saline); CV, coefficient of variation; ns, not significant.

^a,bDifferent letters in the same column and line differ statistically in Tukey’s test.

Table 3. Villus height and crypt depth of jejunum of Ross chicks inoculated at days 1 and 21 of age.

	1 d		21 d
VH, µm	CD, µm	VH, µm	CD, µm
Contamination site
Shell	166.35	67.55	863.34	162.53
Internal	164.67	64.50	941.24	166.81
Inoculated agent
SE	168.10	80.50^a	890.38	182.14^a
NC	162.68	51.50^b	914.21	147.20^b
Variation factor, %
V	ns	ns	ns	ns
A	ns	0.01	ns	0.001
V ×A	ns	ns	ns	ns
CV, %	24.75	42.84	24.88	23.19

VH, villus height; CD, crypt depth; V, contamination site: internal, allantoidal cavity; A, inoculated agent; SE, Salmonella Enteritidis; NC, negative control (0.85% sterilised buffered saline); CV, coefficient of variation; ns, not significant.

^a,bDifferent letters in the same column and line differ statistically in Tukey’s test.

Table 4. Villus height and crypt depth of ileum of Ross chicks at 1 and 21 days of age after inoculation.

	Ileum
	1 d		21 d
	VH, µm	CD, µm	VH, µm	CD, µm
Contamination site
Shell	158.11	71.58	550.70	141.64
Internal	162.01	64.18	577.46	176.11
Inoculated agent
SE	153.00^b	76.70	559.20	193.77
NC	166.43^a	59.18	568.95	123.99
Variation factor, %
V	ns	ns	ns	0.001
A	3.60	0.03	ns	0.001
V x A	ns	0.02	ns	0.12
CV, %	32.62	38.43	18.35	23.67

VH, villus height; CD, crypt depth; V, contamination site: internal, allantoidal cavity; A, inoculated agent; SE, Salmonella Enteritidis; NC, negative control (0.85% sterilised buffered saline); CV, coefficient of variation; ns, not significant.

^a,bDifferent letters in the same column and line differ statistically in Tukey’s test.

Table 5. Mean weight and feed conversion of Ross chicks at days 1, 7, 14 and 21 of age.

	MW1	MW7	MW14	MW21	FC7	FC14	FC21
Contamination site
Shell	47.04	144.83	322.53^a	586.37	1.04	1.16^a	1.55^a
Internal	46.65	145.43	304.24^b	512.23	1.12	1.79^b	1.83^b
Inoculated agent
SE	46.74	145.25	274.70^b	490.51	1.07	2.21^b	1.84^b
NC	46.95	145.01	352.06^a	608.09	1.08	1.29^a	1.55^a
Variation factor, %
V	ns	ns	2.60	0.22	ns	0.63	0.001
A	ns	ns	0.001	0.01	ns	0.01	0.01
V ×A	ns	ns	ns	3.98	ns	ns	ns
CV, %	2.08	6.22	5.31	11.08	4.21	10.16	12.01

MW1, mean weight at one day of age; MW7, mean weight at seven days of age; MW14, mean weight at 14 days of age; MW21, mean weight at 21 days of age; FC7, feed conversion at seven days of age; FC14, feed conversion at 14 days of age; FC21, feed conversion at 21 days of age; SE, Salmonella Enteritidis; NC, negative control (0.85% sterilised buffered saline); A, inoculated agent; V, contamination site: internal, allantoidal cavity; CV, coefficient of variation; ns, not significant.

^a,bDifferent letters in the same column and line differ statistically in Tukey’s test.

Table 6. Bacteriological results of 16 caecal samples from 1-, 7-, 14- and 21-day-old Ross chicks hatched from eggs inoculated with Salmonella Enteritidis.

	1 day	7 days	14 days	21 days	Total positive
Contamination site^#
Shell	2/2	1/2	2/2	2/2	7/8
Internal	2/2	2/2	2/2	½	7/8
Total positive	4/4	¾	4/4	¾	14/16(87.50%)

^#Internal, allantoidal cavity.