Genetic Variability at α s₂-casein Gene in Girgentana Dairy Goat Breed

Figures & data

Table 1. Primers sequences, annealing temperature and reference.

Name	Direction	Sequence	Ta, °C	Reference
B1Z	Forward	5’-CTATCAGATCATCTAGTGAG-3’	53	Vacca et al. (2009a, 2009b)
B1Y	Reverse	5’-CTCTGGGGCAACTTT-3’	53	Vacca et al. (2009a, 2009b)
B1X	Reverse	5’-CTCTGGGGCAACTTC-3’	53	Vacca et al. (2009a, 2009b)
C2Z	Forward	5’-CTGAAGAAGAAAAGAATCGCC-3’	53	Vacca et al. (2009a, 2009b)
C2X	Reverse	5’-CTGGTAATACTGGCTGATTT-3’	53	Vacca et al. (2009a, 2009b)
C2Y	Reverse	5’-CTGGTAATACTGGCTGATTA3’	53	Vacca et al. (2009a, 2009b)
CASDf	Forward	5’- GACACATAGAGAAGATTC-3’	51	Ramunno et al. (2001a)
CASDr	Reverse	5’- CGTTGGGACATTTTATCT-3’	51	Ramunno et al. (2001a)
C16 Fw	Forward	5’- CTGTTTGGTATCATTTAGAATTTAT-3’	56	Chessa et al. (2008)
E16 Rv	Reverse	5’- CTCTTTTTATTACAAAAGACAACTT-3’	56	Chessa et al. (2008)
CASFf	Forward	5’- TCTCTTGCCATCAAAACA-3’	54	Ramunno et al. (2001a)
CASFr	Reverse	5’- TGGTCTTTATTCCTCTCT-3’	54	Ramunno et al. (2001a)

Ta, annealing temperature.

Table 2. Genotype and allele frequencies at locus in Girgentana goat breed.

Genotype	N.	Frequency	Allele	Frequency
AA	106	0.512	A	0.722
AC	17	0.082	C	0.051
AF	70	0.338	E	0.002
CF	4	0.020	F	0.225
EF	1	0.005
FF	9	0.043

N, number of individuals.

Vacca G.M. Ouled Ahmed Ben Ali H. CarcangiuV. PazzolaM. Dettori M.L. 2009a. Genetic structure of the casein gene cluster in the Tunisian native goat breed. Small Ruminant Res. 87 :33-38.

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Vacca G.M. Ouled Ahmed Ben AliH. PazzolaM. SannaM. Dettori M.L. CarcangiuV. 2009b. An investigation on allele frequency at CSN1S2 locus and its relationship with milk parameters in the Sarda goat. J. Anim. Feed Sci. 18 :628-637.

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RamunnoL. CosenzaG. PappalardoM. LongobardiE. GalloD. PastoreN. Di Gregorio P. RandoA. 2001a. Characterization of two new alleles at the goat CSN1S2 locus. Anim. Genet. 32 :264-268.

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ChessaS. RignaneseD. ChiattiF. RadeghieriA. GigliottiC. CaroliA. 2008. Technical note: simultaneous identification of CSN1S2 A, B, C, and E alleles in goat by polymerase chain reaction-single strand conformation polymorphism. J. Dairy Sci. 91 :1214-1217.

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